Any feedback?
Literature summary for 1.1.5.14 extracted from
- Enzymatic studies on the oxidation of sugar and sugar alcohol. I. Purification and properties of particle-bound fructose dehydrogenase (1967), J. Biochem., 61, 636-646.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Gluconobacter cerinus |  |
| Atebrine | slight | Gluconobacter cerinus | |
| Cd2+ | - |
Gluconobacter cerinus | |
| Cu2+ | - |
Gluconobacter cerinus | |
| EDTA | slight | Gluconobacter cerinus | |
| Hg2+ | - |
Gluconobacter cerinus | |
| o-phenanthroline | slight | Gluconobacter cerinus | |
| p-chloromercuribenzoate | - |
Gluconobacter cerinus | |
| Phenylmercuric nitrate | - |
Gluconobacter cerinus | |
| Zn2+ | - |
Gluconobacter cerinus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
D-fructose | in presence of 2,6-dichlorophenolindophenol, 67 mM, pH 5.5, 15°C | Gluconobacter cerinus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | particulate fraction | Gluconobacter cerinus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter cerinus | - |
ASAI IFO 3267 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gluconobacter cerinus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Gluconobacter cerinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + 2,6-dichlorophenolindophenol | most effective electron acceptor | Gluconobacter cerinus | 5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| additional information | no activity with NADP+ | Gluconobacter cerinus | ? | - |
? | |
| additional information | no activity with NAD+ | Gluconobacter cerinus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min, no loss of activity | Gluconobacter cerinus |
| 45 | - |
10 min, 15% loss of activity | Gluconobacter cerinus |
| 50 | - |
10 min, complete loss of activity | Gluconobacter cerinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 5 | - |
Gluconobacter cerinus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.4 | pH 4.5: about 85% of maximal activity, pH 6.4: about 50% of maximal activity | Gluconobacter cerinus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 8.5 | immediately inactivated below pH 2.5 and above 8.5 | Gluconobacter cerinus |
| 5 | 7 | 1 h, 30°C, stable | Gluconobacter cerinus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
