Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
L-2-hydroxyglutarate | at pH 7.4 and 30°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-hydroxyglutarate + a quinone | Arabidopsis thaliana | - |
2-oxoglutarate + a quinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia-0 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
520 | - |
at pH 7.4 and 30°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-2-hydroxyglutarate + 2,6-dichlorophenolindophenol | 3.7% activity compared to L-2-hydroxyglutarate | Arabidopsis thaliana | 2-oxoglutarate + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-2-hydroxyglutarate + 2,6-dichlorophenolindophenol | - |
Arabidopsis thaliana | 2-oxoglutarate + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-2-hydroxyglutarate + a quinone | - |
Arabidopsis thaliana | 2-oxoglutarate + a quinol | - |
? | |
additional information | the enzyme is not active in the presence of NAD+, NADP+, cytochrome c or O2. No activity with glycolate, L-lactate or D-lactate | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
L-2-hydroxyglutarate dehydrogenase | - |
Arabidopsis thaliana |
L-2HG dehydrogenase | - |
Arabidopsis thaliana |
L-2HGDH | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
613 | - |
L-2-hydroxyglutarate | at pH 7.4 and 30°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | more than 50% activity between pH 6.5 and 8.5 | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains FAD. The addition of 2.5 mM FAD results in 31% increase of activity as measured without FAD | Arabidopsis thaliana |