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Literature summary for 1.1.3.B5 extracted from

  • Ewing, T.A.; van Noord, A.; Paul, C.E.; van Berkel, W.J.H.
    A xylenol orange-based screening assay for the substrate specificity of flavin-dependent para-phenol oxidases (2018), Molecules, 23, 164.
    View publication on PubMed
Search for more literature for 1.1.3.B5

Application

Application Comment Organism
analysis development of a screening assay for the substrate specificity of para-phenol oxidases based on the detection of hydrogen peroxide using the ferric-xylenol orange complex method Rhodococcus jostii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodococcus jostii

Protein Variants

Protein Variants Comment Organism
G392F mutant does not display measurable activity Rhodococcus jostii
I427T mutant does not display measurable activity Rhodococcus jostii
L381W mutant does not display measurable activity Rhodococcus jostii
L438C variant displays lowered activity towards all substrates as compared to wild-type Rhodococcus jostii
M282L substrate specificity profile is similar to wild-type Rhodococcus jostii
Q425T display similar activity to the wild-type enzyme with vanillyl alcohol, but no measurable activity towards any other substrate Rhodococcus jostii
V436I variant displays lowered activity towards vanillyl alcohol and eugenol Rhodococcus jostii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
 Vanillyl alcohol His-tagged recombinant enzyme, pH 7.5, 25°C Rhodococcus jostii

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii Q0SBK1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-cyclohexylphenol + O2
-
 Rhodococcus jostii ?
-
 ?
4-cyclopentylphenol + O2
-
 Rhodococcus jostii 4-(1-cyclopenten-1-yl)phenol
-
 ?
eugenol + O2 + H2O
-
 Rhodococcus jostii coniferyl alcohol + H2O2
-
 ?
additional information EUGO displays a somewhat narrower substrate specificity than vanillyl-alcohol oxidase, EC 1.1.3.38. Although alcohols and 4-allylphenols are good substrates for the enzyme, 4-alkylphenols and ethers are hardly accepted Rhodococcus jostii ?
-
 ?
vanillyl alcohol + O2
-
 Rhodococcus jostii vanillin + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
EUGO
-
 Rhodococcus jostii
RHA1_ro03282
-
 Rhodococcus jostii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 4-cyclopentylphenol His-tagged recombinant enzyme, pH 7.5, 25°C Rhodococcus jostii
8
-
 Vanillyl alcohol His-tagged recombinant enzyme, pH 7.5, 25°C Rhodococcus jostii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
170
-
 Vanillyl alcohol His-tagged recombinant enzyme, pH 7.5, 25°C Rhodococcus jostii