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Literature summary for 1.1.3.B4 extracted from

  • Lin, S.F.; Chiou, C.M.; Tsai, Y.C.
    Purification and characterization of a glycerol oxidase from Penicillium sp. TS-622 (1996), Enzyme Microb. Technol., 18, 383-387.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Chloramine T 60% inhibition at 1 mM Penicillium sp.
Hg2+ 60% inhibition at 1 mM Penicillium sp.
hydroxylamine 87% inhibition at 1 mM Penicillium sp.
KCN complete inhibition at 1 mM Penicillium sp.
additional information EDTA, phenylglycoxyl, iodoacetic acid, and o-phenanthroline do not show significant inhibitory effects on the enzyme activity Penicillium sp.
NaN3 complete inhibition at 1 mM Penicillium sp.
Tetranitromethane 46% inhibition at 1 mM Penicillium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
Penicillium sp. 9986
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 15% increase of activity in the presence of 1 mM Cu2+ Penicillium sp.
Fe3+ 10% increase of activity in the presence of 1 mM Fe3+ Penicillium sp.
additional information not influenced by Co2+, Mn2+, Mg2+, Pb2+, Ca2+, and Cd2+ Penicillium sp.
Zn2+ 15% increase of activity in the presence of 1 mM Zn2+ Penicillium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
400000
-
gel filtration Penicillium sp.

Organism

Organism UniProt Comment Textmining
Penicillium sp.
-
-
-
Penicillium sp. TS-622
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, Sephadex G-100 gel filtration, Fractogel DEAE-650M column chromatography, Toyopearl phenyl-650M column chromatography, and Ultrogel hydroxyapatite column chromatography Penicillium sp.

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Penicillium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
crude extract, at pH 7.0 and 30°C Penicillium sp.
66.7
-
after 39.2fold purification, at pH 7.0 and 30°C Penicillium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone + O2 132% activity compared to glycerol Penicillium sp. 3-hydroxy-2-oxopropanal + H2O2
-
?
dihydroxyacetone + O2 132% activity compared to glycerol Penicillium sp. TS-622 3-hydroxy-2-oxopropanal + H2O2
-
?
glycerol + O2 100% activity Penicillium sp. glyceraldehyde + H2O2
-
?
glycerol + O2 100% activity Penicillium sp. TS-622 glyceraldehyde + H2O2
-
?
additional information the enzyme does not oxidize 1,2-propanediol, 1,3-butanediol, glyceraldehyde, glycero-3-phosphate, 3-phosphoglyceric acid, methanol, ethanol, n-propanol, iso-propanol, n-butanol, ethylene glycol, D-glucose, D-galactose, and phenol Penicillium sp. ?
-
?
additional information the enzyme does not oxidize 1,2-propanediol, 1,3-butanediol, glyceraldehyde, glycero-3-phosphate, 3-phosphoglyceric acid, methanol, ethanol, n-propanol, iso-propanol, n-butanol, ethylene glycol, D-glucose, D-galactose, and phenol Penicillium sp. TS-622 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Penicillium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 after 1 h of incubation, the enzyme is relatively stable from 20 to 40°C and loses most of its activity over 50°C Penicillium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Penicillium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 6.5 the enzyme is stable in the pH range from 5.5 to 6.5 Penicillium sp.

Cofactor

Cofactor Comment Organism Structure
heme
-
Penicillium sp.