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Literature summary for 1.1.3.8 extracted from

  • Sugisawa, T.; Ojima, S.; Matzinger, P.K.; Hoshino, T.
    Isolation and characterization of a new vitamin C producing enzyme (L-gulono-gamma-lactone dehydrogenase) of bacterial origin (1995), Biosci. Biotechnol. Biochem., 59, 190-196.
No PubMed abstract available
Search for more literature for 1.1.3.8

Inhibitors

Inhibitors Comment Organism Structure
Cu(NO3)2 0.19 mM concentration 12% inhibition, 0.38 mM concentration 76% inhibition and 0.89 mM concentration 100% inhibition Gluconobacter oxydans
CuCl2 * 2H2O 0.19 mM concentration 16% inhibition, 0.38 mM concentration 92% inhibition and 0.89 mM concentration 100% inhibition Gluconobacter oxydans
CuSO4 0.19 mM concentration 20% inhibition, 0.38 mM concentration 80% inhibition and 0.89 mM concentration 100% inhibition Gluconobacter oxydans
Fe2(SO4)3 sub mM concentration slight inhibition Gluconobacter oxydans
MgCl2 * 6H2O sub mM concentration slight inhibition Gluconobacter oxydans
MgSO4 * 7H2O sub mM concentration slight inhibition Gluconobacter oxydans
MnCl2 * 4H2O 0.38 mM and 0.89 mM concentration 100% inhibition Gluconobacter oxydans
MnSO4 * 4-6H2O 0.38 mM and 0.89 mM concentration 100% inhibition Gluconobacter oxydans
additional information not inhibitory are: EDTA, sodium azide, monoiodoacetate, p-chloromercuribenzoate, NaHAsO4, sodium fluoride, KCN, hydroxyl ammonium sulfate, hydroxylamine, hydrazine monohydrate, hydrazinium dichloride, and 1,4-benzoquinone Gluconobacter oxydans
NiSO4 sub mM concentration slight inhibition Gluconobacter oxydans
TiCl2 sub mM concentration slight inhibition Gluconobacter oxydans
ZnCl2 sub mM concentration slight inhibition Gluconobacter oxydans
ZnSO4 sub mM concentration slight inhibition Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Gluconobacter oxydans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16500
-
 alpha,beta,gamma, 1 * 61000 + 1 * 32500 + 1 * 16500, the largest is a flavoprotein, gel filtration with 0.1% SDS in buffer and SDS-PAGE Gluconobacter oxydans
32500
-
 alpha,beta,gamma, 1 * 61000 + 1 * 32500 + 1 * 16500, the largest is a flavoprotein, gel filtration with 0.1% SDS in buffer and SDS-PAGE Gluconobacter oxydans
61000
-
 alpha,beta,gamma, 1 * 61000 + 1 * 32500 + 1 * 16500, the largest is a flavoprotein, gel filtration with 0.1% SDS in buffer and SDS-PAGE Gluconobacter oxydans
110000
-
 gel filtration Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-gulono-1,4-lactone + O2 Gluconobacter oxydans
-
 L-xylo-hex-3-ulonolactone + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
 strain DSM 4025
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gluconobacter oxydans

Reaction

Reaction Comment Organism Reaction ID
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 product isomerizes spontaneously to L-ascorbate Gluconobacter oxydans
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + phenazine methosulfate + O2 23.4% as active as L-gulono-1,4-lactone Gluconobacter oxydans D-glucosone + ?
-
 ?
D-mannose + phenazine methosulfate + O2 6.4% as active as L-gulono-1,4-lactone Gluconobacter oxydans ?
-
 ?
D-xylose + phenazine methosulfate + O2 6.4% as active as L-gulono-1,4-lactone Gluconobacter oxydans D-xylosone + ?
-
 ?
L-glucurono-1,4-lactone + phenazine methosulfate + O2 6.4% as active as L-gulono-1,4-lactone Gluconobacter oxydans ?
-
 ?
L-gulono-1,4-lactone + 2,6-dichlorophenolindophenol + O2 enzyme is a dehydrogenase, not an oxidase Gluconobacter oxydans L-ascorbic acid + ?
-
 ?
L-gulono-1,4-lactone + O2
-
 Gluconobacter oxydans L-xylo-hex-3-ulonolactone + H2O2
-
 ?
L-gulono-1,4-lactone + O2
-
 Gluconobacter oxydans L-xylo-hex-3-ulonolactone + H2O2 product isomerizes spontaneously to L-ascorbate ?
L-gulono-1,4-lactone + phenazine methosulfate + O2 enzyme is a dehydrogenase, not an oxidase Gluconobacter oxydans L-ascorbic acid + ?
-
 ?

Subunits

Subunits Comment Organism
trimer alpha,beta,gamma, 1 * 61000 + 1 * 32500 + 1 * 16500, the largest is a flavoprotein, gel filtration with 0.1% SDS in buffer and SDS-PAGE Gluconobacter oxydans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 55 no detectable optimum Gluconobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 55 53% activity at 55 C Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9.2
-
 Gluconobacter oxydans