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Literature summary for 1.1.3.7 extracted from

  • Hernandez-Ortega, A.; Lucas, F.; Ferreira, P.; Medina, M.; Guallar, V.; Martinez, A.T.
    Modulating O2 reactivity in a fungal flavoenzyme: involvement of aryl-alcohol oxidase Phe-501 contiguous to catalytic histidine (2011), J. Biol. Chem., 286, 41105-41114.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
F501A site-directed mutagenesis, the mutant shows strongly reduced O2 reactivity compared to the wild-type enzyme Pleurotus eryngii
F501W site-directed mutagenesis, the mutant shows a twofold increase in O2 reactivity compared to the wild-type enzyme Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state and transient kinetics of overall reaction, and oxidative and reductive half-reactions, overview Pleurotus eryngii
0.017
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501Y, overall reaction Pleurotus eryngii
0.029
-
4-methoxybenzyl alcohol pH 6.0, 25°C, wild-type enzyme, overall reaction Pleurotus eryngii
0.046
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501W, oxidative half-reaction Pleurotus eryngii
0.134
-
4-methoxybenzyl alcohol pH 6.0, 25°C, wild-type enzyme, oxidative half-reaction Pleurotus eryngii
0.167
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501A, overall reaction Pleurotus eryngii
0.18
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501Y, oxidative half-reaction Pleurotus eryngii
0.249
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501W, overall reaction Pleurotus eryngii
3.6
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501A, oxidative half-reaction Pleurotus eryngii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-methoxybenzyl alcohol + O2 Pleurotus eryngii
-
4-methoxybenzaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii O94219
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methoxybenzyl alcohol + O2
-
Pleurotus eryngii 4-methoxybenzaldehyde + H2O2
-
?

Synonyms

Synonyms Comment Organism
AAO
-
Pleurotus eryngii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pleurotus eryngii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
40
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501A, overall reaction Pleurotus eryngii
64
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501W, overall reaction Pleurotus eryngii
87
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501Y, overall reaction Pleurotus eryngii
105
-
4-methoxybenzyl alcohol pH 6.0, 25°C, wild-type enzyme, overall reaction Pleurotus eryngii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Pleurotus eryngii

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pleurotus eryngii

General Information

General Information Comment Organism
additional information AAO shows a buried active site connected to the solvent by a hydrophobic funnel-shaped channel, with Phe501 and two other aromatic residues forming a narrow bottleneck that prevents the direct access of alcohol substrates, while O2 has access to the active site following this channel. The side chain of Phe501, contiguous to the catalytic His502 in AAO, helps to position O2 at an adequate distance from flavin C4a (and His502Nepsilon). Phe501 substitution with a bulkier tryptophan residue results in an increase in theO2 reactivity of this flavoenzyme, free diffusion simulations of O2 inside the active-site cavity of AAO, the O2 reactivity of AAO decreases when the access channel is enlarged and increases when it is constricted by introducing a tryptophan residue, overview Pleurotus eryngii
physiological function aryl-alcohol oxidase is a flavoenzyme responsible for activation of O2 to H2O2 in fungal degradation of lignin Pleurotus eryngii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501A, oxidative half-reaction Pleurotus eryngii
240
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501A, overall reaction Pleurotus eryngii
257
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501W, overall reaction Pleurotus eryngii
483
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501Y, oxidative half-reaction Pleurotus eryngii
784
-
4-methoxybenzyl alcohol pH 6.0, 25°C, wild-type enzyme, oxidative half-reaction Pleurotus eryngii
1390
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501W, oxidative half-reaction Pleurotus eryngii
3620
-
4-methoxybenzyl alcohol pH 6.0, 25°C, wild-type enzyme, overall reaction Pleurotus eryngii
5120
-
4-methoxybenzyl alcohol pH 6.0, 25°C, mutant F501Y, overall reaction Pleurotus eryngii