Protein Variants | Comment | Organism |
---|---|---|
F501A | site-directed mutagenesis, the mutant shows strongly reduced O2 reactivity compared to the wild-type enzyme | Pleurotus eryngii |
F501W | site-directed mutagenesis, the mutant shows a twofold increase in O2 reactivity compared to the wild-type enzyme | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state and transient kinetics of overall reaction, and oxidative and reductive half-reactions, overview | Pleurotus eryngii | |
0.017 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501Y, overall reaction | Pleurotus eryngii | |
0.029 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, wild-type enzyme, overall reaction | Pleurotus eryngii | |
0.046 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501W, oxidative half-reaction | Pleurotus eryngii | |
0.134 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, wild-type enzyme, oxidative half-reaction | Pleurotus eryngii | |
0.167 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501A, overall reaction | Pleurotus eryngii | |
0.18 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501Y, oxidative half-reaction | Pleurotus eryngii | |
0.249 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501W, overall reaction | Pleurotus eryngii | |
3.6 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501A, oxidative half-reaction | Pleurotus eryngii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methoxybenzyl alcohol + O2 | Pleurotus eryngii | - |
4-methoxybenzaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | O94219 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methoxybenzyl alcohol + O2 | - |
Pleurotus eryngii | 4-methoxybenzaldehyde + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAO | - |
Pleurotus eryngii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pleurotus eryngii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501A, overall reaction | Pleurotus eryngii | |
64 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501W, overall reaction | Pleurotus eryngii | |
87 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501Y, overall reaction | Pleurotus eryngii | |
105 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, wild-type enzyme, overall reaction | Pleurotus eryngii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Pleurotus eryngii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pleurotus eryngii |
General Information | Comment | Organism |
---|---|---|
additional information | AAO shows a buried active site connected to the solvent by a hydrophobic funnel-shaped channel, with Phe501 and two other aromatic residues forming a narrow bottleneck that prevents the direct access of alcohol substrates, while O2 has access to the active site following this channel. The side chain of Phe501, contiguous to the catalytic His502 in AAO, helps to position O2 at an adequate distance from flavin C4a (and His502Nepsilon). Phe501 substitution with a bulkier tryptophan residue results in an increase in theO2 reactivity of this flavoenzyme, free diffusion simulations of O2 inside the active-site cavity of AAO, the O2 reactivity of AAO decreases when the access channel is enlarged and increases when it is constricted by introducing a tryptophan residue, overview | Pleurotus eryngii |
physiological function | aryl-alcohol oxidase is a flavoenzyme responsible for activation of O2 to H2O2 in fungal degradation of lignin | Pleurotus eryngii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501A, oxidative half-reaction | Pleurotus eryngii | |
240 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501A, overall reaction | Pleurotus eryngii | |
257 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501W, overall reaction | Pleurotus eryngii | |
483 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501Y, oxidative half-reaction | Pleurotus eryngii | |
784 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, wild-type enzyme, oxidative half-reaction | Pleurotus eryngii | |
1390 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501W, oxidative half-reaction | Pleurotus eryngii | |
3620 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, wild-type enzyme, overall reaction | Pleurotus eryngii | |
5120 | - |
4-methoxybenzyl alcohol | pH 6.0, 25°C, mutant F501Y, overall reaction | Pleurotus eryngii |