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Literature summary for 1.1.3.6 extracted from

  • Doukyu, N.; Ishikawa, M.
    Cholesterol oxidase from Rhodococcus erythropolis with high specificity toward beta-cholestanol and pytosterols (2020), PLoS ONE, 15, e0241126 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
EDTA activates Rhodococcus erythropolis
Emal 20CM activates Rhodococcus erythropolis
Sarcosyl activates Rhodococcus erythropolis
sodium cholate activates Rhodococcus erythropolis
Triton X-100 activates Rhodococcus erythropolis
Triton X-405 activates Rhodococcus erythropolis
Tween 20 activates Rhodococcus erythropolis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodococcus erythropolis

Inhibitors

Inhibitors Comment Organism Structure
chloroform
-
Rhodococcus erythropolis
laurylbenzenesulfonate 0.5% (w/v), complete inactivation; 0.5% (w/v), complete inactivation Rhodococcus erythropolis
SDS 0.5% (w/v), complete inactivation; 0.5% (w/v), complete inactivation Rhodococcus erythropolis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0221
-
cholesterol pH 7.0, 30°C Rhodococcus erythropolis
0.0226
-
cholesterol pH 7.0, 30°C Rhodococcus erythropolis

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ 1 mM, 1 h 30°C, partially reduced the activity to 88% Rhodococcus erythropolis
Cu2+ 1 mM, 1 h 30°C, partially reduced the activity to 62% Rhodococcus erythropolis
Cu2+ 1 mM, 1 h 30°C, partially reduced the activity to 88% Rhodococcus erythropolis
Mn2+ 1 mM, 1 h 30°C, partially reduced the activity to 63% Rhodococcus erythropolis
Zn2+ 1 mM, 1 h 30°C, partially reduced the activity to 73% Rhodococcus erythropolis
Zn2+ 1 mM, 1 h 30°C, partially reduced the activity to 79% Rhodococcus erythropolis

Organic Solvent Stability

Organic Solvent Comment Organism
Emal 20CM stable and partially or slightly activated in the presence of 0.5% (w/v) detergent Rhodococcus erythropolis
sarcosyl stable and partially or slightly activated in the presence of 0.5% (w/v) detergent Rhodococcus erythropolis
sodium cholate stable and partially or slightly activated in the presence of 0.5% (w/v) detergent Rhodococcus erythropolis
Triton X-100 stable and partially or slightly activated in the presence of 0.5% (w/v) detergent Rhodococcus erythropolis
Triton X-405 stable and partially or slightly activated in the presence of 0.5% (w/v) detergent Rhodococcus erythropolis
Tween 20 stable and partially or slightly activated in the presence of 0.5% (w/v) detergent Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis C0ZNA9
-
-
Rhodococcus erythropolis C0ZPW5
-
-
Rhodococcus erythropolis PR4 C0ZNA9
-
-
Rhodococcus erythropolis PR4 C0ZPW5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
Rhodococcus erythropolis cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Rhodococcus erythropolis PR4 cholest-5-en-3-one + H2O2
-
?

Synonyms

Synonyms Comment Organism
ChoRI
-
Rhodococcus erythropolis
ChoRII
-
Rhodococcus erythropolis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Rhodococcus erythropolis
55
-
-
Rhodococcus erythropolis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 60% of maximal activity, 50°C: about 60% of maximal activity Rhodococcus erythropolis
40 60 40°C: about 40% of maximal activity, 60°C: about 80% of maximal activity Rhodococcus erythropolis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 30 min, stable Rhodococcus erythropolis
30 60 30 min, stable Rhodococcus erythropolis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.61
-
cholesterol pH 7.0, 30°C Rhodococcus erythropolis
6.8
-
cholesterol pH 7.0, 30°C Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Rhodococcus erythropolis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 pH 5.5: about 50% of maximal activity, pH 8.5: about 55% of maximal activity Rhodococcus erythropolis
5.5 8.5 pH 5.5: about 60% of maximal activity, pH 8.5: about 65% of maximal activity Rhodococcus erythropolis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10.5 ChoRII is stable. Shows higher stability than ChoRI even at pH 11 to 12 Rhodococcus erythropolis
4.5 10 stable Rhodococcus erythropolis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
30
-
cholesterol pH 7.0, 30°C Rhodococcus erythropolis
300
-
cholesterol pH 7.0, 30°C Rhodococcus erythropolis