Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | activates | Rhodococcus erythropolis | |
Emal 20CM | activates | Rhodococcus erythropolis | |
Sarcosyl | activates | Rhodococcus erythropolis | |
sodium cholate | activates | Rhodococcus erythropolis | |
Triton X-100 | activates | Rhodococcus erythropolis | |
Triton X-405 | activates | Rhodococcus erythropolis | |
Tween 20 | activates | Rhodococcus erythropolis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chloroform | - |
Rhodococcus erythropolis | |
laurylbenzenesulfonate | 0.5% (w/v), complete inactivation; 0.5% (w/v), complete inactivation | Rhodococcus erythropolis | |
SDS | 0.5% (w/v), complete inactivation; 0.5% (w/v), complete inactivation | Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0221 | - |
cholesterol | pH 7.0, 30°C | Rhodococcus erythropolis | |
0.0226 | - |
cholesterol | pH 7.0, 30°C | Rhodococcus erythropolis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 1 h 30°C, partially reduced the activity to 88% | Rhodococcus erythropolis | |
Cu2+ | 1 mM, 1 h 30°C, partially reduced the activity to 62% | Rhodococcus erythropolis | |
Cu2+ | 1 mM, 1 h 30°C, partially reduced the activity to 88% | Rhodococcus erythropolis | |
Mn2+ | 1 mM, 1 h 30°C, partially reduced the activity to 63% | Rhodococcus erythropolis | |
Zn2+ | 1 mM, 1 h 30°C, partially reduced the activity to 73% | Rhodococcus erythropolis | |
Zn2+ | 1 mM, 1 h 30°C, partially reduced the activity to 79% | Rhodococcus erythropolis |
Organic Solvent | Comment | Organism |
---|---|---|
Emal 20CM | stable and partially or slightly activated in the presence of 0.5% (w/v) detergent | Rhodococcus erythropolis |
sarcosyl | stable and partially or slightly activated in the presence of 0.5% (w/v) detergent | Rhodococcus erythropolis |
sodium cholate | stable and partially or slightly activated in the presence of 0.5% (w/v) detergent | Rhodococcus erythropolis |
Triton X-100 | stable and partially or slightly activated in the presence of 0.5% (w/v) detergent | Rhodococcus erythropolis |
Triton X-405 | stable and partially or slightly activated in the presence of 0.5% (w/v) detergent | Rhodococcus erythropolis |
Tween 20 | stable and partially or slightly activated in the presence of 0.5% (w/v) detergent | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | C0ZNA9 | - |
- |
Rhodococcus erythropolis | C0ZPW5 | - |
- |
Rhodococcus erythropolis PR4 | C0ZNA9 | - |
- |
Rhodococcus erythropolis PR4 | C0ZPW5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Rhodococcus erythropolis | cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Rhodococcus erythropolis PR4 | cholest-5-en-3-one + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChoRI | - |
Rhodococcus erythropolis |
ChoRII | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Rhodococcus erythropolis |
55 | - |
- |
Rhodococcus erythropolis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30°C: about 60% of maximal activity, 50°C: about 60% of maximal activity | Rhodococcus erythropolis |
40 | 60 | 40°C: about 40% of maximal activity, 60°C: about 80% of maximal activity | Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30 min, stable | Rhodococcus erythropolis |
30 | 60 | 30 min, stable | Rhodococcus erythropolis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.61 | - |
cholesterol | pH 7.0, 30°C | Rhodococcus erythropolis | |
6.8 | - |
cholesterol | pH 7.0, 30°C | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodococcus erythropolis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | pH 5.5: about 50% of maximal activity, pH 8.5: about 55% of maximal activity | Rhodococcus erythropolis |
5.5 | 8.5 | pH 5.5: about 60% of maximal activity, pH 8.5: about 65% of maximal activity | Rhodococcus erythropolis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10.5 | ChoRII is stable. Shows higher stability than ChoRI even at pH 11 to 12 | Rhodococcus erythropolis |
4.5 | 10 | stable | Rhodococcus erythropolis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
cholesterol | pH 7.0, 30°C | Rhodococcus erythropolis | |
300 | - |
cholesterol | pH 7.0, 30°C | Rhodococcus erythropolis |