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Literature summary for 1.1.3.6 extracted from

  • Chernov, K.G.; Neuvonen, M.; Brock, I.; Ikonen, E.; Verkhusha, V.V.
    Introducing inducible fluorescent split cholesterol oxidase to mammalian cells (2017), J. Biol. Chem., 292, 8811-8822 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
diagnostics the enzyme is an important biotechnological tool for clinical diagnostics. It is also used for tracking intracellular cholesterol. Its utility is limited by the lack of an efficient temporal control of its activity. A rapamycin-inducible fluorescent cholesterol oxidase from Chromobacterium sp. DS-1 (split GFP-COase) is engineered. Induction by rapamycin allows temporal control over enzyme activity and rapidly leads to an efficient reduction of intracellular cholesterol. It is proposed that this drug-inducible split GFP-COase is a new tool to manipulate the cellular cholesterol content and gain insights into cholesterol-dependent proteins and cholesterol-related cell pathologies Chromobacterium sp. DS-1
synthesis the enzyme is used for the production of steroid drugs and insecticides Chromobacterium sp. DS-1

Organism

Organism UniProt Comment Textmining
Chromobacterium sp. DS-1 B5MGF8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
Chromobacterium sp. DS-1 cholest-5-en-3-one + H2O2
-
?

Synonyms

Synonyms Comment Organism
COase
-
Chromobacterium sp. DS-1

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first step in the biodegradation of cholesterol Chromobacterium sp. DS-1