Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Chromobacterium sp. |
Crystallization (Comment) | Organism |
---|---|
crystal screen based on sparse matrix formulation, using 500 mM ammonium sulfate, 1.0 M lithium sulfate and 10 mM sodium citrate | Chromobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium sp. | B5MGF8 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography | Chromobacterium sp. |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Chromobacterium sp. | cholest-4-en-3-one + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CHOLOX | belongs to the type II cholesterol oxidases | Chromobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the FAD cofactor is covalently attached to the protein | Chromobacterium sp. |