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Literature summary for 1.1.3.6 extracted from

  • Doukyu, N.; Shibata, K.; Ogino, H.; Sagermann, M.
    Purification and characterization of Chromobacterium sp. DS-1 cholesterol oxidase with thermal, organic solvent, and detergent tolerance (2008), Appl. Microbiol. Biotechnol., 80, 59-70.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ 1 mM, 34% loss of activity Chromobacterium sp. DS-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
cholesterol
-
Chromobacterium sp. DS-1

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Chromobacterium sp. DS-1
-
-

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, 90% loss of activity Chromobacterium sp. DS-1
benzene 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
Butanol 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
chloroform 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
cyclooctane 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
dimethyl sulfoxide 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
Ethanol 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
Ethyl acetate 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
isopropanol 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
Methanol 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
p-xylene 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1
toluene 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable Chromobacterium sp. DS-1

Organism

Organism UniProt Comment Textmining
Chromobacterium sp. DS-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Chromobacterium sp. DS-1

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
Chromobacterium sp. DS-1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.9
-
-
Chromobacterium sp. DS-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-cholestanol + O2 95% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
?
beta-sitosterol + O2 80% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
?
beta-stigmasterol + O2 36% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
?
cholesterol + O2
-
Chromobacterium sp. DS-1 cholest-4-en-3-one + H2O2
-
?
dehydroepiandrosterone + O2 27% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
?
epiandrosterone + O2 15% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
?
ergosterol + O2 12% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
?
additional information no activity with epicholesterol Chromobacterium sp. DS-1 ?
-
?
pregnenolone + O2 95% of the activity with cholesterol Chromobacterium sp. DS-1 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Chromobacterium sp. DS-1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: about 70% of maximal activity, 80°C: about 40% of maximal activity Chromobacterium sp. DS-1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
240 min, enzyme retains 99% of its activity Chromobacterium sp. DS-1
60
-
240 min, enzyme retains 87% of its activity Chromobacterium sp. DS-1
80
-
60 min, the enzyme retains 90% of its activity Chromobacterium sp. DS-1
85
-
30 min, 20% loss of activity Chromobacterium sp. DS-1
90
-
30 min, 72% loss of activity Chromobacterium sp. DS-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Chromobacterium sp. DS-1

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 pH 6.0: about 65% of maximal activity, pH 9.5: about 60% of maximal activcity Chromobacterium sp. DS-1

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 30°C, 1 h, stable Chromobacterium sp. DS-1

Cofactor

Cofactor Comment Organism Structure
FAD enzyme contains 1 mol of FAD per mole of protein Chromobacterium sp. DS-1