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Literature summary for 1.1.3.6 extracted from

  • Singh, S.; Singhal, R.; Malhotra, B.D.
    Immobilization of cholesterol esterase and cholesterol oxidase onto sol-gel films for application to cholesterol biosensor (2007), Anal. Chim. Acta, 582, 335-343.
    View publication on PubMed

Application

Application Comment Organism
analysis cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) can be immobilized onto tetraethylorthosilicate derived sol-gel films. These tetraethylorthosilicate sol-gel/ChEt/ChOx enzyme films can be used for the estimation of cholesterol oleate up to 780 mg/dl (mM). These TEOS sol-gel enzyme films provide selectivity towards glucose, lactate, uric acid and ascorbic acid Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
Pseudomonas sp. cholest-4-en-3-one + H2O2
-
?

Synonyms

Synonyms Comment Organism
ChOx
-
Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
tetraethylorthosilicate sol-gel/ChEt/ChOx (cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) immobilized onto tetraethylorthosilicate derived sol-gel film) reveal thermal stability up to 55°C Pseudomonas sp.