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Literature summary for 1.1.3.6 extracted from

  • Isobe, K.; Mori, N.; Wakao, N.
    The second cholesterol oxidase produced by gamma-Proteobacterium Y-134 (2003), J. Biosci. Bioeng., 96, 257-261.
    View publication on PubMed

General Stability

General Stability Organism
stable in nonionic detergents over a wide range. More than 90% of activity remains after 60 min at 60°C, at concentrations of 0.1% and 0.5% of emulgen A-60, Triton X-100, Triton X-405 or sodium cholate gamma proteobacterium Y-134

Inhibitors

Inhibitors Comment Organism Structure
2-pyridylhydroxymethane sulfate 1 mM, 30 min at 4°C, 32% inhibition gamma proteobacterium Y-134
8-hydroxyquinoline 1 mM, 30 min at 4°C, 20% inhibition gamma proteobacterium Y-134
CoCl2 1 mM, 30 min at 4°C, 21% inhibition gamma proteobacterium Y-134
CuCl2 1 mM, 30 min at 4°C, 29% inhibition gamma proteobacterium Y-134
FAD 1 mM, 30 min at 4°C, 21% inhibition gamma proteobacterium Y-134
FMN 1 mM, 30 min at 4°C, 21% inhibition gamma proteobacterium Y-134
iodoacetic acid 1 mM, 30 min at 4°C, 15% inhibition gamma proteobacterium Y-134
MgCl2 1 mM, 30 min at 4°C, 23% inhibition gamma proteobacterium Y-134
MnCl2 1 mM, 30 min at 4°C, 35% inhibition gamma proteobacterium Y-134
NEM 1 mM, 30 min at 4°C, 24% inhibition gamma proteobacterium Y-134
o-phenanthroline 1 mM, 30 min at 4°C, 26% inhibition gamma proteobacterium Y-134
potassium cyanide 1 mM, 30 min at 4°C, 15% inhibition gamma proteobacterium Y-134
riboflavin 1 mM, 30 min at 4°C, 20% inhibition gamma proteobacterium Y-134
rotenone 1 mM, 30 min at 4°C, 12% inhibition gamma proteobacterium Y-134

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
cholesterol pH 6.0 or pH 7.0 gamma proteobacterium Y-134

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
gel filtration gamma proteobacterium Y-134

Organism

Organism UniProt Comment Textmining
gamma proteobacterium Y-134
-
uncultured
-

Purification (Commentary)

Purification (Comment) Organism
enzyme CHO-U gamma proteobacterium Y-134

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39.6
-
-
gamma proteobacterium Y-134

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-dehydrocholesterol + O2 26% of the activity with cholesterol gamma proteobacterium Y-134 cholest-4,7-dien-3-one + H2O2
-
?
beta-sitosterol + O2 19% of the activity with cholesterol gamma proteobacterium Y-134 stigmast-5-en-3-one + H2O2
-
?
cholesterol + O2
-
gamma proteobacterium Y-134 cholest-4-en-3-one + H2O2
-
?
dihydrocholesterol + O2 22% of the activity with cholesterol gamma proteobacterium Y-134 5alpha-cholestan-3-one + H2O2
-
?
additional information less than 3% of the activity with cholesterol: ergosterol and epiandrosterone. No activity: dehydroepiandrosterone, pregnenolone gamma proteobacterium Y-134 ?
-
?
stigmastanol + O2 7% of the activity with cholesterol gamma proteobacterium Y-134 stigmastanone + H2O2
-
?
stigmasterol + O2 15% of the activity with cholesterol gamma proteobacterium Y-134 stigmasta-5,22-dien-3-one + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 58000, SDS-PAGE gamma proteobacterium Y-134

Synonyms

Synonyms Comment Organism
CHO-U
-
gamma proteobacterium Y-134

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
pH 6.0, 7 h, approximately 65% loss of activity of CHO-U gamma proteobacterium Y-134
70
-
pH 6.0, 1 h, 40% loss of activity of CHO-U gamma proteobacterium Y-134

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
gamma proteobacterium Y-134

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5 pH 5.0: about 90% of maximal activity, pH 6.5: about 60% of maximal activity, pH 7.0: about 20% of maximal activity gamma proteobacterium Y-134

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8.5 60 min, more than 80% of the CHO-U activity remains gamma proteobacterium Y-134

Cofactor

Cofactor Comment Organism Structure
FAD
-
gamma proteobacterium Y-134

pI Value

Organism Comment pI Value Maximum pI Value
gamma proteobacterium Y-134 isoelectric fucusing, pH-gradient 3.5-10.0
-
7