KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
cholestanol | - |
Brevibacterium sterolicum | |
0.07 | - |
cholesterol | - |
Brevibacterium sterolicum | |
0.11 | - |
cholesterol | - |
Brevibacterium sterolicum | |
0.165 | - |
O2 | - |
Brevibacterium sterolicum | |
0.2 | - |
cholesterol | - |
Streptomyces hygroscopicus | |
0.2 | - |
pregnenolone | - |
Streptomyces hygroscopicus | |
0.3 | - |
3beta-hydroxy-androst-5-en-17-one | - |
Streptomyces hygroscopicus | |
0.4 | - |
pregnenolone | - |
Brevibacterium sterolicum | |
0.5 | - |
3beta-hydroxy-androst-5-en-17-one | - |
Streptomyces hygroscopicus | |
0.53 | - |
O2 | - |
Streptomyces hygroscopicus | |
0.7 | - |
cholestanol | - |
Streptomyces hygroscopicus | |
0.8 | - |
cholesterol | - |
Streptomyces hygroscopicus | |
0.8 | - |
3beta-hydroxy-androst-5-en-17-one | - |
Brevibacterium sterolicum | |
1.2 | - |
3beta-hydroxy-androst-5-en-17-one | - |
Brevibacterium sterolicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Streptomyces hygroscopicus | - |
cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Brevibacterium sterolicum | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sterolicum | - |
- |
- |
Streptomyces hygroscopicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Streptomyces hygroscopicus | |
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Brevibacterium sterolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-propanol + O2 | - |
Streptomyces hygroscopicus | propanal + H2O2 | - |
? | |
cholestanol + O2 | - |
Streptomyces hygroscopicus | ? | - |
? | |
cholestanol + O2 | - |
Brevibacterium sterolicum | ? | - |
? | |
cholesterol + O2 | specific for steroid substrates with an OH group in position 3, preference for steroids with at least a C2 chain at position C17 | Streptomyces hygroscopicus | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | specific for steroid substrates with an OH group in position 3, preference for steroids with at least a C2 chain at position C17 | Brevibacterium sterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Streptomyces hygroscopicus | cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Brevibacterium sterolicum | cholest-5-en-3-one + H2O2 | - |
? | |
cyclohexanol + O2 | - |
Streptomyces hygroscopicus | cyclohexanal + H2O2 | - |
? | |
epiandrosterone + O2 | - |
Streptomyces hygroscopicus | ? | - |
? | |
ethanol + O2 | - |
Streptomyces hygroscopicus | ethanal + H2O2 | - |
? | |
methanol + O2 | - |
Streptomyces hygroscopicus | methanal + H2O2 | - |
? | |
pregnenolone + O2 | - |
Streptomyces hygroscopicus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
3beta-hydroxy-androst-5-en-17-one | product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer | Brevibacterium sterolicum | |
0.8 | - |
3beta-hydroxy-5-androsten-17-one | H2O2 production assayed, 100 mM phosphate buffer | Brevibacterium sterolicum | |
7 | - |
epiandrosterone | H2O2 production assayed, 100 mM phosphate buffer | Streptomyces hygroscopicus | |
8.2 | - |
3beta-hydroxy-androst-5-en-17-one | product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer | Streptomyces hygroscopicus | |
21 | - |
pregnenolone | product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer | Brevibacterium sterolicum | |
24 | - |
pregnenolone | product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer | Streptomyces hygroscopicus | |
28 | - |
cholesterol | 50 mM phosphate buffer, pH 7.5 | Streptomyces hygroscopicus | |
37 | - |
cholestanol | H2O2 production assayed, 100 mM phosphate buffer | Streptomyces hygroscopicus | |
40 | - |
cholestanol | H2O2 production assayed, 100 mM phosphate buffer | Brevibacterium sterolicum | |
48 | - |
cholesterol | 50 mM phosphate buffer, pH 7.5 | Brevibacterium sterolicum | |
48.1 | - |
cholesterol | product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer | Brevibacterium sterolicum | |
63 | - |
cholesterol | product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer | Streptomyces hygroscopicus | |
105 | - |
cholesterol | 50 mM phosphate buffer, pH 7.5, 1% thesit, 10% 2-propanol | Brevibacterium sterolicum | |
250 | - |
5-Cholesten-3-one | isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 500 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol | Brevibacterium sterolicum | |
330 | - |
5-Cholesten-3-one | isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 500 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol | Streptomyces hygroscopicus | |
345 | - |
cholesterol | 50 mM phosphate buffer, pH 7.5, 1% thesit, 10% 2-propanol | Streptomyces hygroscopicus | |
670 | - |
5-Cholesten-3-one | isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 50 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol | Streptomyces hygroscopicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | non-covalently bound | Streptomyces hygroscopicus | |
FAD | covalently bound in His-tagged recombinant enzyme | Brevibacterium sterolicum |