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Literature summary for 1.1.3.6 extracted from

  • Fukuyama, M.; Miyake, Y.
    Purification and some properties of cholesterol oxidase from Schizophyllum commune with covalently bound flavin (1979), J. Biochem., 85, 1183-1193.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 inactivates at a concentration of 0.02% Schizophyllum commune

Inhibitors

Inhibitors Comment Organism Structure
sodium deoxycholate
-
Schizophyllum commune
Triton X-100 inactivates at a concentration of 0.02% Schizophyllum commune

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
cholesterol
-
Schizophyllum commune

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
SDS-PAGE, sedimentation equilibrium, amino acid analysis Schizophyllum commune

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Schizophyllum commune
-
cholest-5-en-3-one + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Schizophyllum commune
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Schizophyllum commune

Reaction

Reaction Comment Organism Reaction ID
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Schizophyllum commune

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
Schizophyllum commune cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Schizophyllum commune cholest-5-en-3-one + H2O2
-
?
dehydroepiandrosterone + O2
-
Schizophyllum commune androst-5-en-3,17-dione + H2O2
-
?
lanosterol + O2 slow rate Schizophyllum commune lanosta-8,24-dien-3-one + H2O2
-
?
pregnenolone + O2
-
Schizophyllum commune 5-pregnen-3,20-dione + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 53000, SDS-PAGE Schizophyllum commune

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5
-
Schizophyllum commune

Cofactor

Cofactor Comment Organism Structure
FAD firmly bound Schizophyllum commune
FAD flavoprotein Schizophyllum commune