Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | inactivates at a concentration of 0.02% | Schizophyllum commune |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sodium deoxycholate | - |
Schizophyllum commune | |
Triton X-100 | inactivates at a concentration of 0.02% | Schizophyllum commune |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
cholesterol | - |
Schizophyllum commune |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
SDS-PAGE, sedimentation equilibrium, amino acid analysis | Schizophyllum commune |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Schizophyllum commune | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizophyllum commune | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Schizophyllum commune |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Schizophyllum commune |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Schizophyllum commune | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Schizophyllum commune | cholest-5-en-3-one + H2O2 | - |
? | |
dehydroepiandrosterone + O2 | - |
Schizophyllum commune | androst-5-en-3,17-dione + H2O2 | - |
? | |
lanosterol + O2 | slow rate | Schizophyllum commune | lanosta-8,24-dien-3-one + H2O2 | - |
? | |
pregnenolone + O2 | - |
Schizophyllum commune | 5-pregnen-3,20-dione + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 53000, SDS-PAGE | Schizophyllum commune |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | - |
Schizophyllum commune |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | firmly bound | Schizophyllum commune | |
FAD | flavoprotein | Schizophyllum commune |