Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + O2 | Ptilophora subcostata | - |
D-galactono-1,5-lactone + H2O2 | - |
? | |
D-glucose + O2 | Ptilophora subcostata | - |
D-glucono-1,5-lactone + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ptilophora subcostata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by ammonium sulfate fractionation, followed by anion exchange chromatography and gel filtration | Ptilophora subcostata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
H2O2 producing activities in different algaeal species, and with different substrates, overview | Ptilophora subcostata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + O2 | - |
Ptilophora subcostata | ? + H2O2 | - |
? | |
D-galactose + O2 | - |
Ptilophora subcostata | D-galactono-1,5-lactone + H2O2 | - |
? | |
D-galactose + O2 | best substrate | Ptilophora subcostata | D-galactono-1,5-lactone + H2O2 | - |
? | |
D-glucose + O2 | - |
Ptilophora subcostata | D-glucono-1,5-lactone + H2O2 | - |
? | |
lactose + O2 | - |
Ptilophora subcostata | ? + H2O2 | - |
? | |
maltose + O2 | - |
Ptilophora subcostata | ? + H2O2 | - |
? | |
additional information | ferrous oxidation-xylenol orange (FOX) assay | Ptilophora subcostata | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000 + x * 40000, two bands from purified enzyme in SDS-PAGE, the 29 kDa form seems to be the active/mature enzyme form | Ptilophora subcostata |
Synonyms | Comment | Organism |
---|---|---|
Hox | - |
Ptilophora subcostata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ptilophora subcostata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | purified native enzyme, pH 7.2, 10 min, completely stable at | Ptilophora subcostata |
60 | 70 | purified native enzyme, pH 7.2, 10 min, 50% activity is retained | Ptilophora subcostata |
80 | - |
purified native enzyme, pH 7.2, 10 min, inactivation | Ptilophora subcostata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Ptilophora subcostata |
General Information | Comment | Organism |
---|---|---|
physiological function | hexose oxidase-mediated hydrogen peroxide is the mechanism for the antibacterial activity, e.g. against Bacillus subtilis strain ATCC 6633, in the red seaweed Ptilophora subcostata. Crude algaeal extract prevents the growth of gram-positive and -negative bacteria, overview, which is completely inhibited by treatment with catalase. But the extract does not affect the growth of either a yeast or a filamentous fungus, such as Candida albicans and Penicillium decambens. The activity is highest in the presence of galactose. Enzyme HOX-mediated H2O2 production represents a defense mechanism against a broad range of bacteria in marine algae | Ptilophora subcostata |