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Literature summary for 1.1.3.47 extracted from

  • Feldman, D.; Kowbel, D.J.; Glass, N.L.; Yarden, O.; Hadar, Y.
    Detoxification of 5-hydroxymethylfurfural by the Pleurotus ostreatus lignolytic enzymes aryl alcohol oxidase and dehydrogenase (2015), Biotechnol. Biofuels, 8, 63 .
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
biofuel production current large-scale pretreatment processes for lignocellulosic biomass are generally accompanied by the formation of toxic degradation products, such as 5-hydroxymethylfurfural (HMF), which inhibit cellulolytic enzymes and fermentation by ethanol-producing yeast. Overcoming these toxic effects is a key technical barrier in the biochemical conversion of plant biomass to biofuels. Pleurotus ostreatus, a white-rot fungus, can efficiently degrade lignocellulose, and it can tolerate and metabolize HMF involving HMF oxidase (HMFO) encoded by HmfH Pleurotus ostreatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pleurotus ostreatus
-
-
extracellular the enzyme is secreted Pleurotus ostreatus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,5-diformylfuran + 2 O2 Pleurotus ostreatus
-
 2,5-furandicarboxylic acid + H2O2
-
 ?
2,5-diformylfuran + 2 O2 Pleurotus ostreatus PC9
-
 2,5-furandicarboxylic acid + H2O2
-
 ?
5-hydroxymethylfurfural + O2 Pleurotus ostreatus
-
 2,5-diformylfuran + H2O2
-
 ?
5-hydroxymethylfurfural + O2 Pleurotus ostreatus PC9
-
 2,5-diformylfuran + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus E5CZV8
-
-
Pleurotus ostreatus PC9 E5CZV8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-diformylfuran + 2 O2
-
 Pleurotus ostreatus 2,5-furandicarboxylic acid + H2O2
-
 ?
2,5-diformylfuran + 2 O2
-
 Pleurotus ostreatus PC9 2,5-furandicarboxylic acid + H2O2
-
 ?
5-hydroxymethylfurfural + O2
-
 Pleurotus ostreatus 2,5-diformylfuran + H2O2
-
 ?
5-hydroxymethylfurfural + O2
-
 Pleurotus ostreatus PC9 2,5-diformylfuran + H2O2
-
 ?
5-hydroxymethylfurfural + O2
-
 Pleurotus ostreatus 2,5-furandicarboxylic acid + ?
-
 ?
5-hydroxymethylfurfural + O2
-
 Pleurotus ostreatus PC9 2,5-furandicarboxylic acid + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Pleurotus ostreatus

Synonyms

Synonyms Comment Organism
AAO
-
 Pleurotus ostreatus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pleurotus ostreatus

Expression

Organism Comment Expression
Pleurotus ostreatus 5-hydroxymethylfurfural increases the expression, translation, and activity of enzymes involved in the ligninolytic system, including aryl-alcohol oxidases and a dehydrogenase up
Pleurotus ostreatus expression is induced in presence of 5-hydroxymethylfurfural up

General Information

General Information Comment Organism
evolution the enzyme is a member of the glucosemethanol-choline oxidase (GMC) oxidoreductase superfamily and the AAO family. Phylogenetic analysis of the AAOs in Pleurotus ostreatous revealed that they cluster into two groups Pleurotus ostreatus
physiological function the enzyme is involved in the degradation of 5-hydroxymethylfurfural. Two enzymes groups, which belong to the ligninolytic system, aryl-alcohol oxidase and a dehydrogenase, are involved in this process Pleurotus ostreatus
physiological function Pleurotus ostreatus is capable to metabolize and detoxify 5-hydroxymethylfurfural at 30 mM within 48 h, converting it into 2,5-bis-hydroxymethylfuran and 2,5-furandicarboxylic acid. Two enzymes groups, which belong to the ligninolytic system, aryl-alcohol oxidases and a dehydrogenase, are involved in this process. 5-Hydroxymethylfurfural induces the transcription and production of these enzymes, accompanied by an increase in activity levels Pleurotus ostreatus