Application | Comment | Organism |
---|---|---|
biofuel production | current large-scale pretreatment processes for lignocellulosic biomass are generally accompanied by the formation of toxic degradation products, such as 5-hydroxymethylfurfural (HMF), which inhibit cellulolytic enzymes and fermentation by ethanol-producing yeast. Overcoming these toxic effects is a key technical barrier in the biochemical conversion of plant biomass to biofuels. Pleurotus ostreatus, a white-rot fungus, can efficiently degrade lignocellulose, and it can tolerate and metabolize HMF involving HMF oxidase (HMFO) encoded by HmfH | Pleurotus ostreatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pleurotus ostreatus | - |
- |
extracellular | the enzyme is secreted | Pleurotus ostreatus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-diformylfuran + 2 O2 | Pleurotus ostreatus | - |
2,5-furandicarboxylic acid + H2O2 | - |
? | |
2,5-diformylfuran + 2 O2 | Pleurotus ostreatus PC9 | - |
2,5-furandicarboxylic acid + H2O2 | - |
? | |
5-hydroxymethylfurfural + O2 | Pleurotus ostreatus | - |
2,5-diformylfuran + H2O2 | - |
? | |
5-hydroxymethylfurfural + O2 | Pleurotus ostreatus PC9 | - |
2,5-diformylfuran + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus ostreatus | E5CZV8 | - |
- |
Pleurotus ostreatus PC9 | E5CZV8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-diformylfuran + 2 O2 | - |
Pleurotus ostreatus | 2,5-furandicarboxylic acid + H2O2 | - |
? | |
2,5-diformylfuran + 2 O2 | - |
Pleurotus ostreatus PC9 | 2,5-furandicarboxylic acid + H2O2 | - |
? | |
5-hydroxymethylfurfural + O2 | - |
Pleurotus ostreatus | 2,5-diformylfuran + H2O2 | - |
? | |
5-hydroxymethylfurfural + O2 | - |
Pleurotus ostreatus PC9 | 2,5-diformylfuran + H2O2 | - |
? | |
5-hydroxymethylfurfural + O2 | - |
Pleurotus ostreatus | 2,5-furandicarboxylic acid + ? | - |
? | |
5-hydroxymethylfurfural + O2 | - |
Pleurotus ostreatus PC9 | 2,5-furandicarboxylic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, SDS-PAGE | Pleurotus ostreatus |
Synonyms | Comment | Organism |
---|---|---|
AAO | - |
Pleurotus ostreatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pleurotus ostreatus |
Organism | Comment | Expression |
---|---|---|
Pleurotus ostreatus | 5-hydroxymethylfurfural increases the expression, translation, and activity of enzymes involved in the ligninolytic system, including aryl-alcohol oxidases and a dehydrogenase | up |
Pleurotus ostreatus | expression is induced in presence of 5-hydroxymethylfurfural | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the glucosemethanol-choline oxidase (GMC) oxidoreductase superfamily and the AAO family. Phylogenetic analysis of the AAOs in Pleurotus ostreatous revealed that they cluster into two groups | Pleurotus ostreatus |
physiological function | the enzyme is involved in the degradation of 5-hydroxymethylfurfural. Two enzymes groups, which belong to the ligninolytic system, aryl-alcohol oxidase and a dehydrogenase, are involved in this process | Pleurotus ostreatus |
physiological function | Pleurotus ostreatus is capable to metabolize and detoxify 5-hydroxymethylfurfural at 30 mM within 48 h, converting it into 2,5-bis-hydroxymethylfuran and 2,5-furandicarboxylic acid. Two enzymes groups, which belong to the ligninolytic system, aryl-alcohol oxidases and a dehydrogenase, are involved in this process. 5-Hydroxymethylfurfural induces the transcription and production of these enzymes, accompanied by an increase in activity levels | Pleurotus ostreatus |