Literature summary for 1.1.3.46 extracted from

Diez, V.; Loznik, M.; Taylor, S.; Winn, M.; Rattray, N.J.; Podmore, H.; Micklefield, J.; Goodacre, R.; Medema, M.H.; Mueller, U.; Bovenberg, R.; Janssen, D.B.; Takano, E.
Functional exchangeability of oxidase and dehydrogenase reactions in the biosynthesis of hydroxyphenylglycine, a nonribosomal peptide building block (2015), ACS Synth. Biol., 4, 796-807.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
44	-	L-Mandelate	at pH 7.5 and 28°C	Streptomyces coelicolor
64	-	(S)-4-hydroxymandelate	at pH 7.5 and 28°C	Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38200	-	x * 38200, SDS-PAGE	Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-4-hydroxymandelate + O2	Streptomyces coelicolor	-	2-(4-hydroxyphenyl)-2-oxoacetate + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces coelicolor	Q9Z4X8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-4-hydroxymandelate + O2	-	Streptomyces coelicolor	2-(4-hydroxyphenyl)-2-oxoacetate + H2O2	-	?
L-mandelate + O2	-	Streptomyces coelicolor	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 38200, SDS-PAGE	Streptomyces coelicolor

Synonyms

Synonyms	Comment	Organism
HmO	-	Streptomyces coelicolor
hydroxymandelate oxidase	-	Streptomyces coelicolor
Sco3228	locus name	Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.2	-	L-Mandelate	at pH 7.5 and 28°C	Streptomyces coelicolor
3.5	-	(S)-4-hydroxymandelate	at pH 7.5 and 28°C	Streptomyces coelicolor

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Streptomyces coelicolor

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
26.3	-	L-Mandelate	at pH 7.5 and 28°C	Streptomyces coelicolor
55.5	-	(S)-4-hydroxymandelate	at pH 7.5 and 28°C	Streptomyces coelicolor

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Release 2023.1 (January 2023)