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Literature summary for 1.1.3.41 extracted from

  • Winter, R.T.; Heuts, D.P.; Rijpkema, E.M.; van Bloois, E.; Wijma, H.J.; Fraaije, M.W.
    Hot or not? Discovery and characterization of a thermostable alditol oxidase from Acidothermus cellulolyticus 11B (2012), Appl. Microbiol. Biotechnol., 95, 389-403.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.41

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain MC1061 Streptomyces coelicolor
gene aldO, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Acidothermus cellulolyticus

Protein Variants

Protein Variants Comment Organism
E154P site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
E50P site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
K18R/E19R site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
L234R/D235P site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
R232A/P233G site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
R232A/P233G/L234R/D235P site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
T16R/A17P site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
T16R/A17P/K18R/G19R site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetic analysis, overview Streptomyces coelicolor
additional information
-
 additional information steady-state kinetic analysis, overview Acidothermus cellulolyticus
0.007
-
 xylitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
0.32
-
 xylitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
0.44
-
 D-sorbitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.4
-
 D-sorbitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
5.5
-
 D-mannitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
18
-
 (R)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
27
-
 (S)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
36
-
 D-mannitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
68
-
 1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
83
-
 1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
86
-
 (S)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
101
-
 (R)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
270
-
 glycerol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
350
-
 glycerol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46800
-
 about, sequence calculation Acidothermus cellulolyticus
47000
-
 1 * 47000, SDS-PAGE Streptomyces coelicolor
47000
-
 1 * 47000, SDS-PAGE, 1 * 46800, about, sequence calculation Acidothermus cellulolyticus

Organism

Organism UniProt Comment Textmining
Acidothermus cellulolyticus A0LST6
-
-
Acidothermus cellulolyticus 11B A0LST6
-
-
Streptomyces coelicolor Q9ZBU1
-
-
Streptomyces coelicolor A3(2) Q9ZBU1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, factor Xa protease cleavage of the His-tag Acidothermus cellulolyticus
recombinant His6-tagged enzyme from Escherichia coli strain MC1061 Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor ? + H2O2
-
 ?
(R)-1-phenyl-1,2-ethanediol + O2
-
 Acidothermus cellulolyticus ? + H2O2
-
 ?
(R)-1-phenyl-1,2-ethanediol + O2
-
 Acidothermus cellulolyticus 11B ? + H2O2
-
 ?
(R)-1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor A3(2) ? + H2O2
-
 ?
(S)-1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor ? + H2O2
-
 ?
(S)-1-phenyl-1,2-ethanediol + O2
-
 Acidothermus cellulolyticus ? + H2O2
-
 ?
(S)-1-phenyl-1,2-ethanediol + O2
-
 Acidothermus cellulolyticus 11B ? + H2O2
-
 ?
(S)-1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor A3(2) ? + H2O2
-
 ?
1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor ? + H2O2
-
 ?
1-phenyl-1,2-ethanediol + O2
-
 Acidothermus cellulolyticus ? + H2O2
-
 ?
1-phenyl-1,2-ethanediol + O2
-
 Acidothermus cellulolyticus 11B ? + H2O2
-
 ?
1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor A3(2) ? + H2O2
-
 ?
D-mannitol + O2
-
 Streptomyces coelicolor ? + H2O2
-
 ?
D-mannitol + O2
-
 Acidothermus cellulolyticus ? + H2O2
-
 ?
D-sorbitol + O2
-
 Streptomyces coelicolor ? + H2O2
-
 ?
D-sorbitol + O2
-
 Acidothermus cellulolyticus ? + H2O2
-
 ?
glycerol + O2
-
 Streptomyces coelicolor ? + H2O2
-
 ?
glycerol + O2
-
 Acidothermus cellulolyticus ? + H2O2
-
 ?
glycerol + O2
-
 Acidothermus cellulolyticus 11B ? + H2O2
-
 ?
glycerol + O2
-
 Streptomyces coelicolor A3(2) ? + H2O2
-
 ?
xylitol + O2
-
 Streptomyces coelicolor xylose + H2O2
-
 ?
xylitol + O2
-
 Acidothermus cellulolyticus xylose + H2O2
-
 ?
xylitol + O2
-
 Acidothermus cellulolyticus 11B xylose + H2O2
-
 ?
xylitol + O2
-
 Streptomyces coelicolor A3(2) xylose + H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 47000, SDS-PAGE Streptomyces coelicolor
monomer 1 * 47000, SDS-PAGE, 1 * 46800, about, sequence calculation Acidothermus cellulolyticus

Synonyms

Synonyms Comment Organism
AldO
-
 Streptomyces coelicolor
AldO
-
 Acidothermus cellulolyticus
HotAldO
-
 Streptomyces coelicolor
HotAldO
-
 Acidothermus cellulolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Streptomyces coelicolor
25
-
 assay at Acidothermus cellulolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 60 inactivation at 60°C and 50% remaining activity at 55°C Streptomyces coelicolor
25 84 AldO is a highly thermostable enzyme with an unfolding temperature of 84°C, inactivation above, and an activity half-life at 75°C of 112 min Acidothermus cellulolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0004
-
 (S)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
0.008
-
 (S)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
0.1
-
 (R)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
0.1
-
 1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
0.36
-
 1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
0.74
-
 (R)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.2
-
 D-sorbitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.3
-
 glycerol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.6
-
 glycerol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.9
-
 xylitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
2.5
-
 D-mannitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
9.2
-
 D-mannitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
13
-
 xylitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
17
-
 D-sorbitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Streptomyces coelicolor
7.5
-
 assay at Acidothermus cellulolyticus

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound, flavoenzyme Streptomyces coelicolor
FAD covalently bound, flavoenzyme Acidothermus cellulolyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00001
-
 (S)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
0.0001
-
 (S)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
0.0015
-
 1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
0.0043
-
 1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
0.0046
-
 glycerol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
0.0048
-
 glycerol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
0.0056
-
 (R)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
0.0073
-
 (R)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
0.26
-
 D-mannitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
0.45
-
 D-mannitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
2.7
-
 D-sorbitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
12
-
 D-sorbitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
27
-
 xylitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
41
-
 xylitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor