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Literature summary for 1.1.3.4 extracted from

  • Jagathy, K.; Kalpana, K.; Rajeshkumar, S.
    Production, optimization, characterization and immobilization of glucose oxidase from Aspergillus species (2017), Res. J. Pharm. Technol., 10, 1924-1928.
No PubMed abstract available
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Activating Compound

Activating Compound Comment Organism Structure
Ag2+ 1 mM, 90-95% inhibition Aspergillus niger
Co2+ 1 mM, about 90% inhibition Aspergillus niger
Hg2+ 1 mM, about 85% inhibition Aspergillus niger
Ni2+ 1 mM, about 75% inhibition Aspergillus niger
Pb2+ 1 mM, about 25% inhibition Aspergillus niger
Zn2+ 1 mM, about 55% inhibition Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
89.13
-
 beta-D-glucose pH and temperature not specified in the publication Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
CuCl2 1 mM, enhances activity by 25% Aspergillus niger
KCN 1 mM, enhances activity by 15% Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger PIL7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.81
-
 pH and temperature not specified in the publication Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-d-glucose + O2 10% of the activity compared to beta-D-glucose Aspergillus niger ? + H2O2
-
 ?
2-deoxy-d-glucose + O2 10% of the activity compared to beta-D-glucose Aspergillus niger PIL7 ? + H2O2
-
 ?
3,6-methyl-D-glucose + O2 10% of the activity compared to beta-D-glucose Aspergillus niger ? + H2O2
-
 ?
4-deoxy-d-glucose + O2 7% of the activity compared to beta-D-glucose Aspergillus niger ? + H2O2
-
 ?
4-deoxy-d-glucose + O2 7% of the activity compared to beta-D-glucose Aspergillus niger PIL7 ? + H2O2
-
 ?
4-O-methyl-D-glucose + O2 8% of the activity compared to beta-D-glucose Aspergillus niger ? + H2O2
-
 ?
4-O-methyl-D-glucose + O2 8% of the activity compared to beta-D-glucose Aspergillus niger PIL7 ? + H2O2
-
 ?
6-deoxy-d-glucose + O2 12% of the activity compared to beta-D-glucose Aspergillus niger ? + H2O2
-
 ?
6-deoxy-d-glucose + O2 12% of the activity compared to beta-D-glucose Aspergillus niger PIL7 ? + H2O2
-
 ?
beta-D-glucose + O2
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2
-
 Aspergillus niger PIL7 D-glucono-1,5-lactone + H2O2
-
 ?
L-sorbose + O2 15% of the activity compared to beta-D-glucose Aspergillus niger ? + H2O2
-
 ?
mannose + O2 9% of the activity compared to beta-D-glucose Aspergillus niger ? + H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 160000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
GOX
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 90% of maximal activity, 55°C: 90% of maximal activity, 60°C: 33% of maximal activity Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 half-life: 29.64h Aspergillus niger
30
-
 stable for a minimum of 180 min Aspergillus niger
30
-
 half-life: 516.17 h Aspergillus niger
35
-
 half-life: 78.48 h Aspergillus niger
40
-
 half-life: 36.42 h Aspergillus niger
45
-
 half-life: 18.48 h Aspergillus niger
50
-
 half-life: 11.88 h Aspergillus niger
55
-
 half-life: 8.89 h Aspergillus niger
60
-
 half-life: 1.38 h Aspergillus niger
65
-
 half-life: 27.4 min Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 pH 4.5: about 60% of maximal activity, pH 7.0: about 50% of maximal activity Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 180 min, 14% loss of activity Aspergillus niger
5.5
-
 180 min, 13% loss of activity Aspergillus niger
6
-
 180 min, 11% loss of activity Aspergillus niger