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Literature summary for 1.1.3.4 extracted from

  • Xu, G.; Xu, Y.; Li, A.; Chen, T.; Liu, J.
    Enzymatic bioactivity investigation of glucose oxidase modified with hydrophilic or hydrophobic polymers via in situ RAFT polymerization (2017), J. Polym. Sci. A, 55, 1289-1293 .
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
additional information in situ RAFT polymerization of four different monomers including acrylic acid (AA), methyl acrylate (MA), poly (ethylene glycol) acrylate (PEG-A) and tert-butyl acrylate (TBA) are polymerized directly on the surface of enzyme GOx to afford GOx-poly (PEG-A)(GOx-PPEG-A), GOx-poly(MA)(GOx-PMA), GOx-poly(AA)(GOx-PAA), and GOx-poly(TBA)(GOx-PTBA) conjugates, respectively. PAA and PPEG-A represent the hydrophilic polymers, while PMA and PTBA stand for the hydrophobic ones. Higher bioactivity is obtained for GOx modified with hydrophilic polymers compared with that modified with hydrophobic ones. All the tested polymers can enhance the stability of the GOx, while the hydrophobic GOx-polymers conjugates exhibit much better stability than the hydrophilic ones. Method overview Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
native enzyme Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Storage Stability

Storage Stability Organism
native GOx retains 43% of its bioactivity after 10 days and completely loses activity within 30 days. The bioconjugates of GOx, i.e. GOx-PPEG-A, GOx-PAA, GOx-PMA and GOx-PTBA, still retain bioactivity of 57%, 53%, 59%, and 68%, respectively after 10 days. 27%, 26%, 39%, and 37% of their origin bioactivity are still maintained after 30 days, respectively. Their bioactivities are lost completely after 45 days, 50 days, 55 days, and 55 days for GOx-PPEG-A, GOx-PAA, and GOx-PMA and GOx-PTBA, respectively Aspergillus niger

Synonyms

Synonyms Comment Organism
GOX
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
native GOx retains 48% activity after 10 min, the bioactivity is completely lost after 1 h at 70°C. The bioactivity of the enzyme-polymer conjugates (GOx-PPEG-A, GOx-PAA, GOx-PMA and GOx-PTBA) only slowly declines, GOx-PPEG-A, GOx-PAA, GOx-PMA and GOx-PTBA retain about 61%, 67%, 79%, and 73%, after 10 min and 28%, 24%, 35%, and 37% after 1 h, respectively, at 70°C. The four polymers, hydrophilic or hydrophobic, improve the thermal stability of enzyme GOx. Enzyme modified by hydrophobic polymers (PMA and PTBA) exhibit better thermal stability than that modified by hydrophilic ones (PPEG-A and PAA) Aspergillus niger