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Literature summary for 1.1.3.4 extracted from
- Uncovering and redesigning a key amino acid of glucose oxidase for improved biotechnological applications (2013), Electroanalysis, 25, 606-611 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ferrocenemethanol | a redox mediator | Penicillium amagasakiense | |
| Os-(tpy)(MeCOOH-bpy)Cl2 | a redox mediator | Penicillium amagasakiense |
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | te enzyme is used on electrode surfaces of biosensors | Penicillium amagasakiense |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G423D | site-directed mutagenesis, the mutant shows no activity compared to the wild-type enzyme | Penicillium amagasakiense |
| G423D | site-directed mutagenesis, the mutants containing the mutation G423D leads to quadruple mutants that are not able to reconstitute. The mutant enzymes displays a dramatic decrease in activity compared to thré wild-type enzyme | Penicillium amagasakiense |
| K19E | site-directed mutagenesis | Penicillium amagasakiense |
| K23E | site-directed mutagenesis | Penicillium amagasakiense |
| K260E. | site-directed mutagenesis | Penicillium amagasakiense |
| K424E | site-directed mutagenesis, the single mutation results in a significant increase in the current density which becomes 2.4 fold higher than the current obtained for the wild-type | Penicillium amagasakiense |
| K424I | site-directed mutagenesis, the mutation does not significantly affect the enzyme activity | Penicillium amagasakiense |
| K48/50E | site-directed mutagenesis | Penicillium amagasakiense |
| additional information | for use on electrode surfaces, the key amino acid at the entrance of the active site of glucose oxidase from Penicilium amagasakiense, Lys424, Gln75, Gln184, and Gly423, are redesign by nonactive site mutations, leading to enzymatic anodes with 2.4fold higher current densities, making the biosensor more effective. 424 is the key position | Penicillium amagasakiense |
| Q184E | site-directed mutagenesis | Penicillium amagasakiense |
| Q75E | site-directed mutagenesis | Penicillium amagasakiense |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
beta-D-glucose | recombinant wild-type enzyme, pH 7.0, 37°C, with Os-(tpy)(MeCOOH-bpy)Cl2, immobilized enzyme | Penicillium amagasakiense |  |
| 2.6 | - |
beta-D-glucose | recombinant mutant K424E, pH 7.0, 37°C, with Os-(tpy)(MeCOOH-bpy)Cl2, immobilized enzyme | Penicillium amagasakiense | |
| 3.2 | - |
beta-D-glucose | recombinant mutant K424E, pH 7.0, 37°C, with ferrocenemethanol, immobilized enzyme | Penicillium amagasakiense | |
| 3.4 | - |
beta-D-glucose | recombinant mutant K424I, pH 7.0, 37°C, with ferrocenemethanol, immobilized enzyme | Penicillium amagasakiense | |
| 3.8 | - |
beta-D-glucose | recombinant wild-type enzyme, pH 7.0, 37°C, with ferrocenemethanol, immobilized enzyme | Penicillium amagasakiense | |
| 22 | - |
beta-D-glucose | recombinant mutant K424I, pH 7.0, 37°C, with Os-(tpy)(MeCOOH-bpy)Cl2, immobilized enzyme | Penicillium amagasakiense | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucose + O2 | Penicillium amagasakiense | - |
D-glucono-1,5-lactone + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium amagasakiense | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucose + O2 | - |
Penicillium amagasakiense | D-glucono-1,5-lactone + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GOX | - |
Penicillium amagasakiense |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Penicillium amagasakiense |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 238 | - |
beta-D-glucose | recombinant wild-type enzyme, pH 7.0, 37°C, with Os-(tpy)(MeCOOH-bpy)Cl2, immobilized enzyme | Penicillium amagasakiense | |
| 245 | - |
beta-D-glucose | recombinant mutant K424I, pH 7.0, 37°C, with Os-(tpy)(MeCOOH-bpy)Cl2, immobilized enzyme | Penicillium amagasakiense | |
| 250 | - |
beta-D-glucose | recombinant mutant K424E, pH 7.0, 37°C, with Os-(tpy)(MeCOOH-bpy)Cl2, immobilized enzyme | Penicillium amagasakiense | |
| 433 | - |
beta-D-glucose | recombinant mutant K424I, pH 7.0, 37°C, with ferrocenemethanol, immobilized enzyme | Penicillium amagasakiense | |
| 458 | - |
beta-D-glucose | recombinant wild-type enzyme, pH 7.0, 37°C, with ferrocenemethanol, immobilized enzyme | Penicillium amagasakiense | |
| 464 | - |
beta-D-glucose | recombinant mutant K424E, pH 7.0, 37°C, with ferrocenemethanol, immobilized enzyme | Penicillium amagasakiense | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.1 | 7 | assay at | Penicillium amagasakiense |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Penicillium amagasakiense | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | 424 is a key position for enzyme activity of the immobilized enzyme on electrode surfaces, overview | Penicillium amagasakiense |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 95.2 | - |
beta-D-glucose | recombinant wild-type enzyme, pH 7.0, 37°C, with Os-(tpy)(MeCOOH-bpy)Cl2, immobilized enzyme | Penicillium amagasakiense | |
| 96 | - |
beta-D-glucose | recombinant mutant K424E, pH 7.0, 37°C, with Os-(tpy)(MeCOOH-bpy)Cl2, immobilized enzyme | Penicillium amagasakiense | |
| 115 | - |
beta-D-glucose | recombinant wild-type enzyme, pH 7.0, 37°C | Penicillium amagasakiense | |
| 120 | - |
beta-D-glucose | recombinant wild-type enzyme, pH 7.0, 37°C, with ferrocenemethanol, immobilized enzyme | Penicillium amagasakiense | |
| 122 | - |
beta-D-glucose | recombinant mutant K424I, pH 7.0, 37°C, with Os-(tpy)(MeCOOH-bpy)Cl2, immobilized enzyme | Penicillium amagasakiense | |
| 127 | - |
beta-D-glucose | recombinant mutant K424I, pH 7.0, 37°C, with ferrocenemethanol, immobilized enzyme | Penicillium amagasakiense | |
| 145 | - |
beta-D-glucose | recombinant mutant K424E, pH 7.0, 37°C, with ferrocenemethanol, immobilized enzyme | Penicillium amagasakiense | |
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