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Literature summary for 1.1.3.4 extracted from

  • Ritter, D.W.; Roberts, J.R.; McShane, M.J.
    Glycosylation site-targeted PEGylation of glucose oxidase retains native enzymatic activity (2013), Enzyme Microb. Technol., 52, 279-285.
    View publication on PubMed

Application

Application Comment Organism
analysis the enzyme is useful as biosensor for glucose detection Aspergillus niger

Protein Variants

Protein Variants Comment Organism
additional information PEGylation of GOx provides stability against denaturation or hydrolytic cleavage, glycosylation site-targeted PEGylation of glucose oxidase retains native enzymatic activity, bioconjugate's potential of the enzyme in an optical biosensing assay, overview. The bioconjugate is entrapped within a poly(2-hydroxyethyl methacrylate) hydrogel containing an oxygen-sensitive phosphor, and the construct is shown to respond approximately linearly over the physiologically-relevant glucose range, overview Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86400
-
2 * 86400, native enzyme, SDS-PAGE Aspergillus niger
140000
-
native enzyme, gel filtration Aspergillus niger
182200
-
x * 182200, PEGylated enzyme, SDS-PAGE Aspergillus niger
669000
-
PEGylated enzyme, gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation type VII Aspergillus niger
-

Subunits

Subunits Comment Organism
? x * 182200, PEGylated enzyme, SDS-PAGE Aspergillus niger
dimer 2 * 86400, native enzyme, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
GOX
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
loss in specific activity of PEGylated GOx and native GOx in the absence of glucose and at 37°C, the PEGylated enzyme is more stable and retains 62.6% after 24 h and about 40% after 30 days, the native enzyme retains 67.3% after 24 h and about 20% after 30 days, overview Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1
-
assay at Aspergillus niger