Application | Comment | Organism |
---|---|---|
analysis | the enzyme is useful as biosensor for glucose detection | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
additional information | PEGylation of GOx provides stability against denaturation or hydrolytic cleavage, glycosylation site-targeted PEGylation of glucose oxidase retains native enzymatic activity, bioconjugate's potential of the enzyme in an optical biosensing assay, overview. The bioconjugate is entrapped within a poly(2-hydroxyethyl methacrylate) hydrogel containing an oxygen-sensitive phosphor, and the construct is shown to respond approximately linearly over the physiologically-relevant glucose range, overview | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86400 | - |
2 * 86400, native enzyme, SDS-PAGE | Aspergillus niger |
140000 | - |
native enzyme, gel filtration | Aspergillus niger |
182200 | - |
x * 182200, PEGylated enzyme, SDS-PAGE | Aspergillus niger |
669000 | - |
PEGylated enzyme, gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | type VII | Aspergillus niger | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 182200, PEGylated enzyme, SDS-PAGE | Aspergillus niger |
dimer | 2 * 86400, native enzyme, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
GOX | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
loss in specific activity of PEGylated GOx and native GOx in the absence of glucose and at 37°C, the PEGylated enzyme is more stable and retains 62.6% after 24 h and about 40% after 30 days, the native enzyme retains 67.3% after 24 h and about 20% after 30 days, overview | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.1 | - |
assay at | Aspergillus niger |