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Literature summary for 1.1.3.4 extracted from
- Purification and characterization of D-glucose oxidase from white-rot fungus Pleurotus ostreatus (1993), Eur. J. Biochem., 215, 747-752.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCN | substancial stimulation | Pleurotus ostreatus |  |
| p-chloromercuribenzoate | substancial stimulation | Pleurotus ostreatus | |
| Sodium nitrate | substancial stimulation | Pleurotus ostreatus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-hydroxyquinoline | - |
Pleurotus ostreatus | |
| Ag+ | inhibits oxidation of the reduced FAD moiety competing with molecular oxygen as a hydrogen acceptor | Pleurotus ostreatus | |
| Ag2SO4 | - |
Pleurotus ostreatus | |
| HgCl2 | - |
Pleurotus ostreatus | |
| NaN3 | - |
Pleurotus ostreatus | |
| phenylmercuric acetate | - |
Pleurotus ostreatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Pleurotus ostreatus | |
| 1.34 | - |
beta-D-glucose | - |
Pleurotus ostreatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasmic space | - |
Pleurotus ostreatus | 42597 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
4 * 70000, SDS-PAGE | Pleurotus ostreatus |
| 290000 | - |
gel filtration | Pleurotus ostreatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pleurotus ostreatus | important role in lignin-degradation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pleurotus ostreatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ammonium sulfate precipitation, gel filtration, anion-exchange and hydrophobic-interaction chromatography | Pleurotus ostreatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-glucose + O2 + H2O | 38% relative activity to D-glucose | Pleurotus ostreatus | 2-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
| alpha-methyl-D-glucoside + O2 + H2O | 13% relative activity to D-glucose | Pleurotus ostreatus | ? + H2O2 | - |
? | |
| beta-D-glucose + 2,6-dichlorophenol indophenol | - |
Pleurotus ostreatus | D-glucono-1,5-lactone + ? | - |
? | |
| beta-D-glucose + 4-benzoquinone | - |
Pleurotus ostreatus | D-glucono-1,5-lactone + 4-benzoquinol | - |
r | |
| beta-D-glucose + N,N,N',N'-tetramethyl-1,4-phenylenediamine | - |
Pleurotus ostreatus | ? | - |
r | |
| beta-D-glucose + O2 + H2O | 2,6-dichloroindophenol, N,N,N',N'-tetramethyl-1,4-phenylenediamine, and 4-benzoquinone can function as electron acceptors | Pleurotus ostreatus | D-glucono-1,5-lactone + H2O2 | - |
? | |
| beta-D-glucose + O2 + H2O | glucose is the primary substrate for the enzyme | Pleurotus ostreatus | D-glucono-1,5-lactone + H2O2 | - |
? | |
| cellobiose + O2 + H2O | 13% relative activity to D-glucose | Pleurotus ostreatus | ? + H2O2 | - |
? | |
| D-glucono-1,5-lactone + O2 + H2O | 80% relative activity to D-glucose | Pleurotus ostreatus | ? + H2O2 | - |
? | |
| L-gulono-gamma-lactone + O2 + H2O | 62% relative activity to D-glucose | Pleurotus ostreatus | ? + H2O2 | - |
? | |
| L-sorbose + O2 + H2O | 86% relative activity to D-glucose | Pleurotus ostreatus | ? | - |
? | |
| mannose + O2 + H2O | 9% relative activity to D-glucose | Pleurotus ostreatus | ? + H2O2 | - |
? | |
| additional information | important role in lignin-degradation | Pleurotus ostreatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 70000, SDS-PAGE | Pleurotus ostreatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Pleurotus ostreatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable for 120 min | Pleurotus ostreatus |
| 73 | - |
70% loss of activity after 60 min | Pleurotus ostreatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
N,N,N',N'-tetramethyl-1,4-phenylenediamine | - |
Pleurotus ostreatus | |
| 1.13 | - |
2,6-dichloroindophenol | - |
Pleurotus ostreatus | |
| 15.5 | - |
4-benzoquinone | - |
Pleurotus ostreatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Pleurotus ostreatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 4 mol flavin per mol of enzyme | Pleurotus ostreatus | |
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