General Stability | Organism |
---|---|
3°C, little loss of activity, 3 years | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | 153000 | sedimentation and diffusion data | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + O2 + H2O | Aspergillus niger | - |
D-glucono-1,5-lactone + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus niger |
Purification (Comment) | Organism |
---|---|
using dialysis, ammonium sulfate fractionation and column chromatography on DEAE-cellulose | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Aspergillus niger |
Storage Stability | Organism |
---|---|
3°C, purified enzyme, several years | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + O2 + H2O | 20% relative activity to D-glucose | Aspergillus niger | 2-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
3-deoxy-D-glucose + O2 + H2O | 1% relative activity to D-glucose | Aspergillus niger | 3-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
4-deoxy-D-glucose + O2 + H2O | 2% relative activity to D-glucose | Aspergillus niger | 4-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
4-O-methy-D-glucose + O2 + H2O | 15% relative activity to D-glucose | Aspergillus niger | 4-O-methyl-D-glucono-1,5-lactone + H2O2 | - |
? | |
6-deoxy-D-glucose + O2 + H2O | 10% relative activity to D-glucose | Aspergillus niger | 6-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
6-O-methyl-D-glucose + O2 + H2O | 1% relative activity to D-glucose | Aspergillus niger | 6-O-methyl-D-glucono-1,5-lactone + H2O2 | - |
? | |
alpha-D-glucose + O2 + H2O | very slow reaction | Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? | |
beta-D-glucose + O2 + H2O | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? | |
beta-D-glucose + O2 + H2O | highly specific | Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? | |
beta-D-glucose + O2 + H2O | glucose is the primary substrate for the enzyme | Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? | |
mannose + O2 + H2O | 1% relative activity to D-glucose | Aspergillus niger | ? + H2O2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is very stable at cold temperatures | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 2 mol per mol of enzyme | Aspergillus niger |