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Literature summary for 1.1.3.38 extracted from

  • Jin, J.; Mazon, H.; van den Heuvel, R.H.H.; Janssen, D.B.; Fraaije, M.W.
    Discovery of a eugenol oxidase from Rhodococcus sp. strain RHA1 (2007), FEBS J., 274, 2311-2321 .
    View publication on PubMed
Search for more literature for 1.1.3.38

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodococcus jostii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 4-ethylguaiacol pH 7.5, 25°C Rhodococcus jostii
0.0023
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C Rhodococcus jostii
0.04
-
 Vanillyl alcohol pH 7.5, 25°C Rhodococcus jostii
0.076
-
 vanillylamine pH 7.5, 25°C Rhodococcus jostii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
111000
-
 gel filtration Rhodococcus jostii

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii Q0SBK1
-
-

Purification (Commentary)

Purification (Comment) Organism
recpombinant enzyme Rhodococcus jostii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(methoxymethyl)phenol + O2
-
 Rhodococcus jostii ? + H2O2
-
 ?
4-ethylguaiacol + O2
-
 Rhodococcus jostii ? + H2O2
-
 ?
5-indanol + O2
-
 Rhodococcus jostii ? + H2O2
-
 ?
additional information enzyme also oxidizes eugenol. Poor substrates: vanillylamine, 4-ethylguaiacol Rhodococcus jostii ?
-
-
vanillyl alcohol + O2
-
 Rhodococcus jostii vanillin + H2O2
-
 ?
vanillylamine + O2
-
 Rhodococcus jostii ? + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 58000, SDS-PAGE, 2 * 58681, calculated from sequence Rhodococcus jostii

Synonyms

Synonyms Comment Organism
RHA1_ro03282
-
 Rhodococcus jostii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 0.0034 mM enzyme in in 20 mm Tris ? HCl, pH 7.5 for 90 min, no inactivation Rhodococcus jostii
60
-
 half-life 30 min, in presence of 3fold excess of FAD half-life 45 min Rhodococcus jostii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C Rhodococcus jostii
0.026
-
 4-ethylguaiacol pH 7.5, 25°C Rhodococcus jostii
0.26
-
 vanillylamine pH 7.5, 25°C Rhodococcus jostii
12
-
 Vanillyl alcohol pH 7.5, 25°C Rhodococcus jostii

pH Range

pH Minimum pH Maximum Comment Organism
9 10 more than 90% of maximum activity Rhodococcus jostii

Cofactor

Cofactor Comment Organism Structure
FAD eugenol oxidase is partly expressed in the apo form, but can be fully flavinylated by the addition of FAD. FAD is covalently linked to residue H390. Protein shows absorption maxima at 365 nm and 441 nm, and shoulders at 313 nm, 394 nm, and 461 nm Rhodococcus jostii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C Rhodococcus jostii
3.4
-
 vanillylamine pH 7.5, 25°C Rhodococcus jostii
12
-
 4-ethylguaiacol pH 7.5, 25°C Rhodococcus jostii
300
-
 Vanillyl alcohol pH 7.5, 25°C Rhodococcus jostii