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Literature summary for 1.1.3.38 extracted from

  • van den Heuvel, R.H.; van den Berg, W.A.; Rovida, S.; van Berkel, W.J.
    Laboratory-evolved vanillyl-alcohol oxidase produces natural vanillin (2004), J. Biol. Chem., 279, 33492-33500.
    View publication on PubMed
Search for more literature for 1.1.3.38

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes I238T, F454Y, E520G and T505S are overexpressed in Escherichia coli Penicillium simplicissimum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor diffusion method. Crystal structures of mutant enzymes I238T, F454Y, E502G and T505S are highly similar to that of wild-type enzyme Penicillium simplicissimum

Protein Variants

Protein Variants Comment Organism
E502G the octamer/dimer ratio is 1:10. The catalytic efficiency of the mutant is significantly increased for ortho-substituted 4-methylphenols Penicillium simplicissimum
F454Y as for wild-type enzyme the octamer/dimer ratio of the mutant enzyme is 1.5:1. The catalytic efficiency of the mutant is significantly increased for ortho-substituted 4-methylphenols Penicillium simplicissimum
I238T the octamer/dimer ratio is 4:1. The catalytic efficiency of the mutant is significantly increased for ortho-substituted 4-methylphenols Penicillium simplicissimum
T505S as for wild-type enzyme the octamer/dimer ratio of the mutant enzyme is 1.5:1 Penicillium simplicissimum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 eugenol pH 10, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.0008
-
 eugenol pH 10, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.0008
-
 eugenol pH 7.5, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.001
-
 2-methyl-p-cresol pH 10, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.001
-
 creosol pH 10, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.001
-
 creosol pH 10, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.002
-
 eugenol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
0.002
-
 creosol pH 10, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.002
-
 eugenol pH 10, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.002
-
 creosol pH 10, 25°C, wild-type enzyme Penicillium simplicissimum
0.003
-
 eugenol pH 7.5, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.004
-
 4-(Methoxymethyl)phenol pH 10, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.005
-
 eugenol pH 7.5, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.006
-
 2-methyl-p-cresol pH 7.5, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.006
-
 Vanillyl alcohol pH 7.5, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.007
-
 2-methyl-p-cresol pH 7.5, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.007
-
 Vanillyl alcohol pH 7.5, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.007
-
 Vanillyl alcohol pH 7.5, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.007
-
 2-methyl-p-cresol pH 7.5, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.008
-
 eugenol pH 7.5, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.01
-
 Vanillyl alcohol pH 10, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.01
-
 Vanillyl alcohol pH 10, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.013
-
 creosol pH 7.5, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.014
-
 4-(Methoxymethyl)phenol pH 10, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.015
-
 Vanillyl alcohol pH 10, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.017
-
 2-methyl-p-cresol pH 7.5, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.019
-
 eugenol pH 10, 25°C, wild-type enzyme Penicillium simplicissimum
0.02
-
 creosol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
0.02
-
 4-(Methoxymethyl)phenol pH 10, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.021
-
 2-methyl-p-cresol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
0.022
-
 Vanillyl alcohol pH 10, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.023
-
 4-(Methoxymethyl)phenol pH 10, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.027
-
 creosol pH 7.5, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.029
-
 2-amino-p-cresol pH 10, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.031
-
 p-Cresol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
0.031
-
 creosol pH 7.5, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.033
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.041
-
 creosol pH 7.5, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.043
-
 2-amino-p-cresol pH 10, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.05
-
 2-amino-p-cresol pH 10, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.051
-
 Vanillyl alcohol pH 7.5, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.051
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.054
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.058
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
0.064
-
 2-amino-p-cresol pH 10, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.064
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.075
-
 Vanillyl alcohol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
0.11
-
 4-(Methoxymethyl)phenol pH 10, 25°C, wild-type enzyme Penicillium simplicissimum
0.118
-
 2-amino-p-cresol pH 7.5, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.123
-
 2-amino-p-cresol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
0.167
-
 2-amino-p-cresol pH 7.5, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.189
-
 Vanillyl alcohol pH 10, 25°C, wild-type enzyme Penicillium simplicissimum
0.2
-
 2-amino-p-cresol pH 7.5, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.254
-
 2-amino-p-cresol pH 7.5, 25°C, mutant enzyme T505S Penicillium simplicissimum

Organism

Organism UniProt Comment Textmining
Penicillium simplicissimum P56216
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium simplicissimum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-p-cresol + O2
-
 Penicillium simplicissimum ?
-
 ?
2-methyl-p-cresol + O2
-
 Penicillium simplicissimum ?
-
 ?
4-(methoxymethyl)phenol + O2
-
 Penicillium simplicissimum ?
-
 ?
creosol + O2
-
 Penicillium simplicissimum ?
-
 ?
eugenol + O2
-
 Penicillium simplicissimum ?
-
 ?
p-cresol + O2
-
 Penicillium simplicissimum ?
-
 ?
vanillyl alcohol + O2
-
 Penicillium simplicissimum vanillin + H2O2
-
 ?

Subunits

Subunits Comment Organism
More octamer/dimer ratio is 1.5:1 for wild-type enzyme and mutant enzymes F454Y and T505S. For I238T the octamer/dimer ratio is 4:1. For E502G the octamer/dimer ratio is 1:10 Penicillium simplicissimum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 2-amino-p-cresol pH 10, 25°C, wild-type enzyme Penicillium simplicissimum
0.002
-
 2-methyl-p-cresol pH 10, 25°C, wild-type enzyme Penicillium simplicissimum
0.003
-
 creosol pH 8, 25°C, wild-type enzyme Penicillium simplicissimum
0.005
-
 creosol pH 10, 25°C, wild-type enzyme Penicillium simplicissimum
0.01
-
 2-methyl-p-cresol pH 10, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.01
-
 creosol pH 8, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.013
-
 creosol pH 10, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.013
-
 creosol pH 8, 25°C, wild-type enzyme Penicillium simplicissimum
0.015
-
 creosol pH 10, 25°C, mutant enzyme A429V Penicillium simplicissimum
0.015
-
 creosol pH 8, 25°C, mutant enzyme I237T/M437L Penicillium simplicissimum
0.016
-
 creosol pH 8, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.017
-
 2-amino-p-cresol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
0.017
-
 creosol pH 10, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.017
-
 creosol pH 8, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.018
-
 creosol pH 8, 25°C, mutant enzyme A429V Penicillium simplicissimum
0.019
-
 creosol pH 10, 25°C, mutant enzyme F93Y/P189S/I238T Penicillium simplicissimum
0.019
-
 2-methyl-p-cresol pH 7.5, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.02
-
 creosol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
0.02
-
 creosol pH 10, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.02
-
 creosol pH 10, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.02
-
 2-methyl-p-cresol pH 10, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.021
-
 creosol pH 10, 25°C, mutant enzyme I237T/M437L Penicillium simplicissimum
0.022
-
 creosol pH 8, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.023
-
 2-methyl-p-cresol pH 10, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.025
-
 2-methyl-p-cresol pH 7.5, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.028
-
 2-methyl-p-cresol pH 10, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.031
-
 2-methyl-p-cresol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
0.032
-
 2-methyl-p-cresol pH 7.5, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.046
-
 2-amino-p-cresol pH 10, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.047
-
 2-amino-p-cresol pH 10, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.063
-
 2-methyl-p-cresol pH 7.5, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.07
-
 creosol pH 10, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.076
-
 2-amino-p-cresol pH 10, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.079
-
 2-amino-p-cresol pH 7.5, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.082
-
 2-amino-p-cresol pH 7.5, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.086
-
 2-amino-p-cresol pH 10, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.087
-
 2-amino-p-cresol pH 7.5, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.09
-
 creosol pH 10, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.099
-
 2-amino-p-cresol pH 7.5, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.1
-
 eugenol pH 10, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.1
-
 creosol pH 10, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.1
-
 creosol pH 10, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.1
-
 creosol pH 7.5, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.12
-
 creosol pH 7.5, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.14
-
 creosol pH 7.5, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.17
-
 eugenol pH 7.5, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.17
-
 creosol pH 7.5, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.21
-
 Vanillyl alcohol pH 7.5, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.22
-
 Vanillyl alcohol pH 7.5, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.23
-
 eugenol pH 10, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.25
-
 Vanillyl alcohol pH 7.5, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.3
-
 Vanillyl alcohol pH 10, 25°C, mutant enzyme E502G Penicillium simplicissimum
0.39
-
 Vanillyl alcohol pH 7.5, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.5
-
 eugenol pH 7.5, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.55
-
 eugenol pH 10, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.6
-
 eugenol pH 10, 25°C, mutant enzyme I238T Penicillium simplicissimum
0.65
-
 eugenol pH 7.5, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.67
-
 Vanillyl alcohol pH 10, 25°C, mutant enzyme T505S Penicillium simplicissimum
0.71
-
 Vanillyl alcohol pH 10, 25°C, mutant enzyme F454Y Penicillium simplicissimum
0.8
-
 eugenol pH 7.5, 25°C, mutant enzyme I238T Penicillium simplicissimum
1.1
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme E502G Penicillium simplicissimum
1.2
-
 4-(Methoxymethyl)phenol pH 10, 25°C, mutant enzyme E502G Penicillium simplicissimum
1.4
-
 Vanillyl alcohol pH 10, 25°C, mutant enzyme I238T Penicillium simplicissimum
1.6
-
 Vanillyl alcohol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
2.1
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme F454Y Penicillium simplicissimum
2.1
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme I238T Penicillium simplicissimum
2.3
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme T505S Penicillium simplicissimum
2.4
-
 Vanillyl alcohol pH 10, 25°C, wild-type enzyme Penicillium simplicissimum
3
-
 4-(Methoxymethyl)phenol pH 10, 25°C, mutant enzyme I238T Penicillium simplicissimum
3.1
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
3.4
-
 4-(Methoxymethyl)phenol pH 10, 25°C, mutant enzyme F454Y Penicillium simplicissimum
3.6
-
 4-(Methoxymethyl)phenol pH 10, 25°C, mutant enzyme T505S Penicillium simplicissimum
3.9
-
 4-(Methoxymethyl)phenol pH 10, 25°C, wild-type enzyme Penicillium simplicissimum
14
-
 eugenol pH 7.5, 25°C, wild-type enzyme Penicillium simplicissimum
39
-
 eugenol pH 10, 25°C, wild-type enzyme Penicillium simplicissimum