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Literature summary for 1.1.3.20 extracted from
- Purification and some properties of alcohol oxidase from alkane-grown Candida tropicalis (1992), Biochem. J., 282, 325-331.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Detergents | e.g. cholate, activation, purified preparations do not need detergents for activation | Candida tropicalis |  |
General Stability
| General Stability | Organism |
|---|---|
| CHAPS, 0.5%, stabilizes during solubilization | Candida tropicalis |
| cholate, 1%, solubilizes and stabilizes | Candida tropicalis |
| phenylmethylsulfonyl fluoride stabilizes during purification and storage | Candida tropicalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| light | complete inactivation, t1/2: 7 min; photochemical inactivation at wavelength of 450 nm, i.e. blue-light region of the spectrum of visible light | Candida tropicalis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
Tetradecanol | - |
Candida tropicalis | |
| 0.005 | - |
hexadecanol | - |
Candida tropicalis | |
| 0.0061 | - |
Dodecanol | - |
Candida tropicalis | |
| 0.042 | - |
Decanol | - |
Candida tropicalis | |
| 0.061 | - |
O2 | - |
Candida tropicalis | |
| 1 | - |
Octanol | - |
Candida tropicalis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Candida tropicalis | 16020 | - |
| microsome | - |
Candida tropicalis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
2 * 70000, SDS-PAGE | Candida tropicalis |
| 145000 | - |
gel filtration | Candida tropicalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| long-chain alcohol + O2 | Candida tropicalis | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida tropicalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Candida tropicalis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a long-chain alcohol + O2 = a long-chain aldehyde + H2O2 | ping-pong mechanism | Candida tropicalis | |
| a long-chain alcohol + O2 = a long-chain aldehyde + H2O2 | 2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2 | Candida tropicalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Candida tropicalis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, in the dark stable for at least 10 h | Candida tropicalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-tetradecanol + O2 | 75% the rate of dodecanol oxidation | Candida tropicalis | tetradecanal + H2O2 | - |
? | |
| 12-hydroxydodecanoic acid + O2 | poor substrate | Candida tropicalis | ? | - |
? | |
| 2-dodecanol + O2 | - |
Candida tropicalis | ? | - |
? | |
| alpha,omega-alkanediols + O2 | from C10 to C16 | Candida tropicalis | ? | - |
? | |
| decanol + O2 | oxidation at 70% the rate of dodecanol oxidation | Candida tropicalis | decanal + H2O2 | - |
? | |
| dodecanol + O2 | i.e. lauryl alcohol, preferred substrate | Candida tropicalis | dodecanal + H2O2 | - |
? | |
| hexadecanol + O2 | 19% the rate of dodecanol oxidation | Candida tropicalis | hexadecanal + H2O2 | - |
? | |
| long-chain alcohol + O2 | - |
Candida tropicalis | ? | - |
? | |
| additional information | weak activity towards fatty aldehydes | Candida tropicalis | ? | - |
? | |
| additional information | no substrate: dodecan-4-ol, dodecan-5-ol | Candida tropicalis | ? | - |
? | |
| n-octanol + O2 | oxidation at 34% the rate of dodecanol oxidation | Candida tropicalis | n-octanal + H2O2 | - |
? | |
| octadecanol + O2 | oxidation at 12% the rate of dodecanol oxidation | Candida tropicalis | octadecanal + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 70000, SDS-PAGE | Candida tropicalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
inactivation overnight | Candida tropicalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
60% activity at pH 7.0 | Candida tropicalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
inactivation after 3 min | Candida tropicalis |
| 7 | - |
and below unstable | Candida tropicalis |
| 9 | - |
at least 15 min stable | Candida tropicalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | not FAD, FMN, riboflavin, different absorption and fluorescence emission spectra, flavin is probably covalently bound | Candida tropicalis | |
| additional information | no cofactor: riboflavin, 2,6-dichlorophenolindophenol/phenazine methosulfate, potassium ferricyanide, cytochrome c, nitro blue tetrazolium, tetramethyl-p-phenylenediamine | Candida tropicalis |
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