Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.2 extracted from

  • Yorita, K.; Matsuoka, T.; Misaki, H.; Massey, V.
    Interaction of two arginine residues in lactate oxidase with the enzyme flavin conversion of FMN to 8-formyl-FMN (2000), Proc. Natl. Acad. Sci. USA, 97, 13039-13044 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.2

Protein Variants

Protein Variants Comment Organism
R181K mutation in conserved residue, efficiency of reduction of the oxidized flavin by L-lactate is greatly reduced Aerococcus viridans
R181K/R268K mutation in conserved residue, efficiency of reduction of the oxidized flavin by L-lactate is greatly reduced Aerococcus viridans
R181M mutation in conserved residue, efficiency of reduction of the oxidized flavin by L-lactate is greatly reduced Aerococcus viridans
R268K mutation in conserved residue, efficiency of reduction of the oxidized flavin by L-lactate is greatly reduced. Mutation also results in a slow conversion of the 8-CH3-substituent of FMN to yield 8-formyl-FMN Aerococcus viridans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 O2 mutant R181K, pH 7.0, 25°C Aerococcus viridans
0.03
-
 O2 mutant R181M, pH 7.0, 25°C Aerococcus viridans
0.16
-
 O2 wild-type, pH 7.0, 25°C Aerococcus viridans
0.94
-
 L-lactate wild-type, pH 7.0, 25°C Aerococcus viridans
7.5
-
 L-lactate mutant R181K, pH 7.0, 25°C Aerococcus viridans
103
-
 L-lactate mutant R181M, pH 7.0, 25°C Aerococcus viridans

Organism

Organism UniProt Comment Textmining
Aerococcus viridans Q44467
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lactate + O2
-
 Aerococcus viridans pyruvate + H2O2
-
 ?