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Literature summary for 1.1.3.2 extracted from

  • Yorita, K.; Janko, K.; Aki, K.; Ghisla, S.; Palfey, B.A.; Massey, V.
    On the reaction mechanism of L-lactate oxidase quantitative structure-activity analysis of the reaction with para-substituted L-mandelates (1997), Proc. Natl. Acad. Sci. USA, 94, 9590-9595 .
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Aerococcus viridans Q44467
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-hydroxy-2-phenylacetate + O2
-
Aerococcus viridans phenylpyruvate + H2O2
-
?
2-hydroxy-2-(4-chlorophenyl)acetate + O2
-
Aerococcus viridans (4-chlorophenyl)pyruvate + H2O2
-
?
2-hydroxy-2-(4-hydroxyphenyl)acetate + O2
-
Aerococcus viridans (4-hydroxyphenyl)pyruvate + H2O2
-
?
2-hydroxy-2-(4-methoxyphenyl)acetate + O2
-
Aerococcus viridans (4-methoxyphenyl)pyruvate + H2O2
-
?
2-hydroxy-2-(4-methylphenyl)acetate + O2
-
Aerococcus viridans (4-methylphenyl)pyruvate + H2O2
-
?
2-hydroxy-2-(4-nitrophenyl)acetate + O2
-
Aerococcus viridans (4-nitrophenyl)pyruvate + H2O2
-
?
L-alpha-hydroxy-beta-methylvalerate + O2
-
Aerococcus viridans 3-methyl-2-oxopentanoate + H2O2
-
?
additional information mechanism with little development of charge in the transition state, such as a transfer of hydride to the flavin N(5) position or a synchronous mechanism in which the alpha-C-H is formally abstracted as a H+ while the resulting charge is simultaneously neutralized by another event Aerococcus viridans ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.6
-
(S)-2-Hydroxy-2-phenylacetate mutant A95G, pH 7.0, 25°C Aerococcus viridans
12
-
L-alpha-hydroxy-beta-methylvalerate mutant A95G, pH 7.0, 25°C Aerococcus viridans

Cofactor

Cofactor Comment Organism Structure
FMN flavin reduction is a direct measure of alpha-C-H bond breakage Aerococcus viridans