Protein Variants | Comment | Organism |
---|---|---|
A95G | activity with L-lactate similar to wild-type, increased activity with longer chain L-alpha-hydroxyacids such as alpha-hydroxy-n-butyric acid, alpha-hydroxy-n-valeric acid, and also with L-mandelic acid. Reduction of the enzyme bound flavin by substrates is the rate-limiting step in A95G | Aerococcus viridans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
(S)-mandelic acid | wild-type, pH 7.0, 25°C | Aerococcus viridans | |
0.87 | - |
L-lactate | wild-type, pH 7.0, 25°C | Aerococcus viridans | |
5 | - |
glycerate | wild-type, pH 7.0, 25°C | Aerococcus viridans | |
5.5 | - |
2-hydroxypentanoate | wild-type, pH 7.0, 25°C | Aerococcus viridans | |
6.75 | - |
L-lactate | mutant A95G, pH 7.0, 25°C | Aerococcus viridans | |
10 | - |
2-hydroxypentanoate | mutant A95G, pH 7.0, 25°C | Aerococcus viridans | |
18 | - |
2-hydroxybutanoate | wild-type, pH 7.0, 25°C | Aerococcus viridans | |
20 | - |
(S)-mandelic acid | mutant A95G, pH 7.0, 25°C | Aerococcus viridans | |
27 | - |
2-hydroxybutanoate | mutant A95G, pH 7.0, 25°C | Aerococcus viridans | |
53 | - |
glycerate | mutant A95G, pH 7.0, 25°C | Aerococcus viridans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aerococcus viridans | Q44467 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-mandelic acid + O2 | - |
Aerococcus viridans | phenylpyruvate + H2O2 | - |
? | |
2-hydroxybutanoate + O2 | - |
Aerococcus viridans | 2-oxobutanoate + H2O2 | - |
? | |
2-hydroxypentanoate + O2 | - |
Aerococcus viridans | 2-oxopentanoate + H2O2 | - |
? | |
glycerate + O2 | - |
Aerococcus viridans | hydroxypyruvate + H2O2 | - |
? | |
L-lactate + O2 | - |
Aerococcus viridans | pyruvate + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
(S)-mandelic acid | wild-type, pH 7.0, 25°C | Aerococcus viridans | |
0.87 | - |
glycerate | wild-type, pH 7.0, 25°C | Aerococcus viridans | |
1.25 | - |
2-hydroxypentanoate | wild-type, pH 7.0, 25°C | Aerococcus viridans | |
11 | - |
2-hydroxybutanoate | wild-type, pH 7.0, 25°C | Aerococcus viridans | |
15.8 | - |
(S)-mandelic acid | mutant A95G, pH 7.0, 25°C | Aerococcus viridans | |
31.3 | - |
glycerate | mutant A95G, pH 7.0, 25°C | Aerococcus viridans | |
51.7 | - |
2-hydroxypentanoate | mutant A95G, pH 7.0, 25°C | Aerococcus viridans | |
250 | - |
L-lactate | mutant A95G, pH 7.0, 25°C | Aerococcus viridans | |
283 | - |
L-lactate | wild-type, pH 7.0, 25°C | Aerococcus viridans | |
510 | - |
2-hydroxybutanoate | mutant A95G, pH 7.0, 25°C | Aerococcus viridans |