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Literature summary for 1.1.3.2 extracted from
- Purification and properties of Aerococcus viridans lactate oxidase (1989), Biochem. Biophys. Res. Commun., 164, 919-926 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Bromopyruvate | irreversible inactivation | Aerococcus viridans |  |
| D-lactate | competitive | Aerococcus viridans | |
| DL-2-Hydroxybutanoate | competitive | Aerococcus viridans | |
| glycolate | competitive | Aerococcus viridans | |
| oxalate | competitive | Aerococcus viridans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
(S)-lactate | pH 7.0, 37°C | Aerococcus viridans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 162000 | - |
gel filtration | Aerococcus viridans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aerococcus viridans | Q44467 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aerococcus viridans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 272 | - |
pH 7.0, 37°C | Aerococcus viridans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-lactate + O2 | - |
Aerococcus viridans | pyruvate + H2O2 | - |
? | |
| additional information | enzyme is highly specific for L-lactate. No substrates: D-lactate, glycolate and DL-2-hydroxybutanoate | Aerococcus viridans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 44000, SDS-PAGE | Aerococcus viridans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Aerococcus viridans | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
glycolate | pH 7.0, 37°C | Aerococcus viridans | |
| 2.9 | - |
D-lactate | pH 7.0, 37°C | Aerococcus viridans | |
| 5.3 | - |
oxalate | pH 7.0, 37°C | Aerococcus viridans | |
| 15 | - |
DL-2-Hydroxybutanoate | pH 7.0, 37°C | Aerococcus viridans | |
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Release 2023.1 (January 2023)
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