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Literature summary for 1.1.3.18 extracted from

  • Tjallinks, G.; Martin, C.; Fraaije, M.
    Enantioselective oxidation of secondary alcohols by the flavoprotein alcohol oxidase from Phanerochaete chrysosporium (2021), Arch. Biochem. Biophys., 704, 108888 .
    View publication on PubMed
Search for more literature for 1.1.3.18

Application

Application Comment Organism
synthesis enzyme cartalyzes selective oxidation of several secondary (S)-alcohols, so the (R)-alcohols are obtained from racemic mixtures in high enantiopurity Phanerodontia chrysosporium

Crystallization (Commentary)

Crystallization (Comment) Organism
in silico docking studies. Several hydrophobic and aromatic residues in the substrate binding site create a cavity in which the substrates can comfortably undergo van der Waals and pi-stacking interactions. Consequently, oxidation of the secondary alcohols is restricted to one of the two enantiomers Phanerodontia chrysosporium

Protein Variants

Protein Variants Comment Organism
F101S variant is less capable of converting the (S)-alcohols and does not convert any of the (R)-alcohols Phanerodontia chrysosporium
L317F mutant displays low or no significant activity with secondary alcohols and/or lower enantioselectivity than wild-type Phanerodontia chrysosporium
T315S mutant displays low or no significant activity with secondary alcohols and/or lower enantioselectivity than wild-type Phanerodontia chrysosporium
W560F mutant displays low or no significant activity with secondary alcohols and/or lower enantioselectivity than wild-type Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium T2M2J4 cf. EC 1.1.3.13
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-indanol + O2
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 Phanerodontia chrysosporium 1-indanone + H2O2
-
 ?
1-phenylethanol + O2
-
 Phanerodontia chrysosporium 1-phenylthanone + H2O2
-
 ?
2-pentanol + O2
-
 Phanerodontia chrysosporium 2-pentanone + H2O2
-
 ?
3-butyn-2-ol + O2
-
 Phanerodontia chrysosporium 3-butyn-2-one + H2O2
-
 ?
additional information enzyme acts both on secondary alcohols, and on primary alcohols, reaction of EC 1.1.3.13. By selective oxidation of the (S)-alcohols, the (R)-alcohols can be obtained in high enantiopurity Phanerodontia chrysosporium ?
-
-

Synonyms

Synonyms Comment Organism
AOX1
-
 Phanerodontia chrysosporium

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Phanerodontia chrysosporium