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Literature summary for 1.1.3.18 extracted from
- Purification and some properties of polyvinyl alcohol-degrading enzyme produced by Pseudomonas O-3 (1976), Agric. Biol. Chem., 40, 497-504.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-dinitrophenol | activity 101.6% | Pseudomonas sp. |  |
| alpha,alpha'-dipyridyl | activity 102% | Pseudomonas sp. | |
| Monoiodoacetic acid | activity 102.5% | Pseudomonas sp. | |
| Na3AsO3 | activity 101.4% | Pseudomonas sp. | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Pseudomonas sp. | |
| EDTA | - |
Pseudomonas sp. | |
| hydroxylamine | - |
Pseudomonas sp. | |
| Ni2+ | - |
Pseudomonas sp. | |
| salicylaldoxine | - |
Pseudomonas sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | cofactor | Pseudomonas sp. | |
| Fe2+ | cofactor | Pseudomonas sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
gel filtration | Pseudomonas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
O-3 | - |
| Pseudomonas sp. O-3 | - |
O-3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 22.73 | - |
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polyvinyl alcohol + O2 | - |
Pseudomonas sp. | polyvinylketone + H2O2 | - |
? | |
| polyvinyl alcohol + O2 | - |
Pseudomonas sp. O-3 | polyvinylketone + H2O2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudomonas sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | - |
Pseudomonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable up to, activity completely lost at 75°C | Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | stable in this range at 5°C, above pH 11 activity decreases rapidly | Pseudomonas sp. |
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