Cloned (Comment) | Organism |
---|---|
PCR-amplification, expression in Escherichia coli BL21 Star | Streptococcus iniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42380 | - |
calculated, 390 amino acids, 869 bp | Streptococcus iniae |
42384 | - |
1 * 42384, type 1 enzyme | Streptococcus iniae |
44038 | - |
1 * 44038, type 2 enzyme | Streptococcus iniae |
44040 | - |
calculated, 407 amino acids, 920 bp, 51 bp repeat sequence compared to type 1 enzyme | Streptococcus iniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactate + O2 | Streptococcus iniae | - |
pyruvate + H2O2 | - |
? | |
lactate + O2 | Streptococcus iniae QMA0177 | - |
pyruvate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus iniae | A9QH69 | strains QMA0165 (type 1 enzyme) | - |
Streptococcus iniae | A9QH71 | type 2 lactate oxidase isolated from Strptococcus iniae; major pathogen of farmed fish, type 2 is an isolate from diseased barramundi (Lates calcarifer) in Northern Territory, Australia | - |
Streptococcus iniae QMA0177 | A9QH71 | type 2 lactate oxidase isolated from Strptococcus iniae; major pathogen of farmed fish, type 2 is an isolate from diseased barramundi (Lates calcarifer) in Northern Territory, Australia | - |
Purification (Comment) | Organism |
---|---|
centrifugation, washing with 10 mM potassium phosphate buffer, pH 6.5, stored at -20°C, resuspended with same buffer, treated with lysozyme followed by disruption with glass beads in a bead mill, centrifugation, supernatants used for enzyme assays | Streptococcus iniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.07 | - |
type 1 enzyme expressed in Escherichia coli BL21Star, 10 mM potassium phophate, pH 6,5, 0.01 mM flavin adenine nucleotide, 37°C, 0.02% 2,2-azinobis (3-ethylbenzthiazolinesulfonic acid), 10 mM L-(+)-lactate, 0.5 U horseradish peroxidase (peroxidase-coupled spectrophotometric assay) | Streptococcus iniae |
45.76 | - |
type 2 enzyme expressed in Escherichia coli BL21Star, 10 mM potassium phophate, pH 6.5, 0.01 mM flavin adenine nucleotide, 37°C, 0.02% 2,2-azinobis (3-ethylbenzthiazolinesulfonic acid), 10 mM L-(+)-lactate, 0.5 U horseradish peroxidase (peroxidase-coupled spectrophotometric assay) | Streptococcus iniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactate + O2 | - |
Streptococcus iniae | pyruvate + H2O2 | - |
? | |
lactate + O2 | - |
Streptococcus iniae QMA0177 | pyruvate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42384, type 1 enzyme | Streptococcus iniae |
monomer | 1 * 44038, type 2 enzyme | Streptococcus iniae |
Synonyms | Comment | Organism |
---|---|---|
lactate oxidase | flavin mononucleotide-dependent | Streptococcus iniae |
LctO | type 1 enzyme | Streptococcus iniae |
LctO | type 2 enzyme (novel variant of lactate oxidase gene) | Streptococcus iniae |