Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenolindophenol | inhibition by excess substrate | Homo sapiens | |
glycolate | inhibition by excess substrate | Homo sapiens | |
glyoxylate | inhibition by excess substrate | Homo sapiens | |
L-Mandelate | inhibition by excess substrate | Homo sapiens | |
Mandelate | - |
Homo sapiens | |
oxalate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
glycolate | - |
Homo sapiens | |
0.033 | - |
2,6-dichlorophenolindophenol | - |
Homo sapiens | |
0.32 | - |
glycolate | - |
Homo sapiens | |
1.5 | - |
L-Mandelate | - |
Homo sapiens | |
3.4 | - |
glyoxylate | - |
Homo sapiens | |
16.5 | - |
L-lactate | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41290 | - |
determined by mass spectrometry. After tag-removal two close bands appear on SDS-PAGE one at 45.287 kDa and one at 41.288 kDa | Homo sapiens |
45290 | - |
determined by mass spectrometry. After tag-removal two close bands appear on SDS-PAGE one at 45.287 kDa and one at 41.288 kDa | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified in one step on hydroxyapatite column onto which it is strongly adsorbed. For the initial purification steps, FMN is added to the buffers | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-70°C, concentrated solution, stable for several months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + 2,6-dichlorophenolindophenol | - |
Homo sapiens | glyoxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
glyoxylate + 2,6-dichlorophenolindophenol | - |
Homo sapiens | ? | - |
? | |
L-lactate + 2,6-dichlorophenolindophenol | - |
Homo sapiens | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-mandelate + 2,6-dichlorophenolindophenol | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | gel filtration | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
glycolate oxidase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
L-Mandelate | - |
Homo sapiens | |
0.46 | - |
L-lactate | - |
Homo sapiens | |
0.83 | - |
glyoxylate | - |
Homo sapiens | |
3.6 | - |
glycolate | - |
Homo sapiens | |
15.6 | - |
glycolate | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
2,6-dichlorophenolindophenol | - |
Homo sapiens | |
12 | - |
glyoxylate | - |
Homo sapiens | |
36 | - |
glycolate | - |
Homo sapiens | |
46 | - |
glycolate | - |
Homo sapiens | |
92 | - |
L-Mandelate | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1.8 | - |
- |
Homo sapiens | oxalate | |
3.7 | - |
- |
Homo sapiens | Mandelate |