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Literature summary for 1.1.3.15 extracted from

  • Urban, P.; Chirat, I.; Lederer, F.
    Rat kidney L-2-hydroxyacid oxidase. Structural and mechanistic comparison with flavocytochrome b2 from bakerÆs yeast (1988), Biochemistry, 27, 7365-7371.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxybutynoate inhibition of transhydrogenation reaction Rattus sp.
phosphate 50% inhibition at 0.1 M Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
DL-phenyllactate pH 7.0 and 7.5 Rattus sp.
0.15
-
DL-2-hydroxycaproate pH 7.5 Rattus sp.
0.25
-
DL-2-hydroxyvalerate pH 7.5 Rattus sp.
0.25
-
DL-2-hydroxycaproate pH 7.0 Rattus sp.
0.35
-
DL-2-hydroxyvalerate pH 7.0 Rattus sp.
0.4
-
L-Mandelate pH 7.5 Rattus sp.
0.6
-
DL-2-hydroxybutyrate pH 7.0 Rattus sp.
0.7
-
L-2-Hydroxyisocaproate pH 7.5 Rattus sp.
0.7
-
DL-3-chlorolactate pH 7.5 Rattus sp.
0.8
-
L-Mandelate pH 7.0 Rattus sp.
0.8
-
DL-3-chlorolactate pH 7.0 Rattus sp.
0.9
-
L-2-Hydroxyisocaproate pH 7.0 Rattus sp.
1
-
DL-2-hydroxybutyrate pH 7.5 Rattus sp.
1.8
-
L-lactate pH 7.0 Rattus sp.
3.4
-
L-lactate pH 7.5 Rattus sp.
4
-
DL-methionine pH 7.5 Rattus sp.
4.4
-
Bromopyruvate transhydrogenation reaction Rattus sp.
6
-
L-leucine pH 7.5 Rattus sp.
35
-
L-tryptophan pH 7.5 Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DL-2-hydroxybutyrate + O2 Rattus sp.
-
2-oxobutyrate + H2O2
-
?
DL-2-hydroxycaproate + O2 Rattus sp.
-
2-oxocaproate + H2O2
-
?
DL-2-hydroxyvalerate + O2 Rattus sp.
-
2-oxovalerate + H2O2
-
?
DL-methionine + O2 Rattus sp.
-
? + H2O2
-
?
L-2-hydroxyisocaproate + O2 Rattus sp.
-
2-oxoisocaproate + H2O2
-
?
L-lactate + O2 Rattus sp.
-
pyruvate + H2O2
-
?
L-leucine + O2 Rattus sp.
-
? + H2O2
-
?
L-mandelate + O2 Rattus sp.
-
? + H2O2
-
?
L-tryptophan + O2 Rattus sp.
-
? + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 2-step chromatography Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
-
Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxybutanoate + bromopyruvate + ? transhydrogenation reaction Rattus sp. bromolactate + pyruvate + ?
-
?
DL-2-hydroxybutyrate + O2
-
Rattus sp. 2-oxobutyrate + H2O2
-
?
DL-2-hydroxycaproate + O2
-
Rattus sp. 2-oxocaproate + H2O2
-
?
DL-2-hydroxyvalerate + O2
-
Rattus sp. 2-oxovalerate + H2O2
-
?
DL-3-chlorolactate + O2
-
Rattus sp. 3-chloropyruvate + H2O2
-
?
DL-methionine + O2
-
Rattus sp. ? + H2O2
-
?
DL-phenyllactate + O2
-
Rattus sp. phenylpyruvate + H2O2
-
?
L-2-hydroxyisocaproate + O2
-
Rattus sp. 2-oxoisocaproate + H2O2
-
?
L-lactate + O2
-
Rattus sp. pyruvate + H2O2
-
?
L-leucine + O2
-
Rattus sp. ? + H2O2
-
?
L-mandelate + O2
-
Rattus sp. ? + H2O2
-
?
L-tryptophan + O2
-
Rattus sp. ? + H2O2
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.36
-
Bromopyruvate transhydrogenation reaction Rattus sp.

Cofactor

Cofactor Comment Organism Structure
FMN one FAD per subunit Rattus sp.