Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hydroxybutynoate | inhibition of transhydrogenation reaction | Rattus sp. | |
phosphate | 50% inhibition at 0.1 M | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
DL-phenyllactate | pH 7.0 and 7.5 | Rattus sp. | |
0.15 | - |
DL-2-hydroxycaproate | pH 7.5 | Rattus sp. | |
0.25 | - |
DL-2-hydroxyvalerate | pH 7.5 | Rattus sp. | |
0.25 | - |
DL-2-hydroxycaproate | pH 7.0 | Rattus sp. | |
0.35 | - |
DL-2-hydroxyvalerate | pH 7.0 | Rattus sp. | |
0.4 | - |
L-Mandelate | pH 7.5 | Rattus sp. | |
0.6 | - |
DL-2-hydroxybutyrate | pH 7.0 | Rattus sp. | |
0.7 | - |
L-2-Hydroxyisocaproate | pH 7.5 | Rattus sp. | |
0.7 | - |
DL-3-chlorolactate | pH 7.5 | Rattus sp. | |
0.8 | - |
L-Mandelate | pH 7.0 | Rattus sp. | |
0.8 | - |
DL-3-chlorolactate | pH 7.0 | Rattus sp. | |
0.9 | - |
L-2-Hydroxyisocaproate | pH 7.0 | Rattus sp. | |
1 | - |
DL-2-hydroxybutyrate | pH 7.5 | Rattus sp. | |
1.8 | - |
L-lactate | pH 7.0 | Rattus sp. | |
3.4 | - |
L-lactate | pH 7.5 | Rattus sp. | |
4 | - |
DL-methionine | pH 7.5 | Rattus sp. | |
4.4 | - |
Bromopyruvate | transhydrogenation reaction | Rattus sp. | |
6 | - |
L-leucine | pH 7.5 | Rattus sp. | |
35 | - |
L-tryptophan | pH 7.5 | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-2-hydroxybutyrate + O2 | Rattus sp. | - |
2-oxobutyrate + H2O2 | - |
? | |
DL-2-hydroxycaproate + O2 | Rattus sp. | - |
2-oxocaproate + H2O2 | - |
? | |
DL-2-hydroxyvalerate + O2 | Rattus sp. | - |
2-oxovalerate + H2O2 | - |
? | |
DL-methionine + O2 | Rattus sp. | - |
? + H2O2 | - |
? | |
L-2-hydroxyisocaproate + O2 | Rattus sp. | - |
2-oxoisocaproate + H2O2 | - |
? | |
L-lactate + O2 | Rattus sp. | - |
pyruvate + H2O2 | - |
? | |
L-leucine + O2 | Rattus sp. | - |
? + H2O2 | - |
? | |
L-mandelate + O2 | Rattus sp. | - |
? + H2O2 | - |
? | |
L-tryptophan + O2 | Rattus sp. | - |
? + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, 2-step chromatography | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
- |
Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybutanoate + bromopyruvate + ? | transhydrogenation reaction | Rattus sp. | bromolactate + pyruvate + ? | - |
? | |
DL-2-hydroxybutyrate + O2 | - |
Rattus sp. | 2-oxobutyrate + H2O2 | - |
? | |
DL-2-hydroxycaproate + O2 | - |
Rattus sp. | 2-oxocaproate + H2O2 | - |
? | |
DL-2-hydroxyvalerate + O2 | - |
Rattus sp. | 2-oxovalerate + H2O2 | - |
? | |
DL-3-chlorolactate + O2 | - |
Rattus sp. | 3-chloropyruvate + H2O2 | - |
? | |
DL-methionine + O2 | - |
Rattus sp. | ? + H2O2 | - |
? | |
DL-phenyllactate + O2 | - |
Rattus sp. | phenylpyruvate + H2O2 | - |
? | |
L-2-hydroxyisocaproate + O2 | - |
Rattus sp. | 2-oxoisocaproate + H2O2 | - |
? | |
L-lactate + O2 | - |
Rattus sp. | pyruvate + H2O2 | - |
? | |
L-leucine + O2 | - |
Rattus sp. | ? + H2O2 | - |
? | |
L-mandelate + O2 | - |
Rattus sp. | ? + H2O2 | - |
? | |
L-tryptophan + O2 | - |
Rattus sp. | ? + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
Bromopyruvate | transhydrogenation reaction | Rattus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | one FAD per subunit | Rattus sp. |