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Literature summary for 1.1.3.15 extracted from

  • Iwamoto, K.; Ikawa, T.
    A novel glycolate oxidase requiring flavin mononucleotide as the cofactor in the Prasinophycean alga Mesostigma viride (2000), Plant Cell Physiol., 41, 988-991.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-pyridylhydroxymethanesulfonate strong inhibition between 0.1-1 mM Mesostigma viride
8-hydroxyquinoline 50% inhibition at 0.05 mM Mesostigma viride
bipyridine strong inhibition Mesostigma viride
o-phenanthroline strong inhibition between 0.1-1.0 mM Mesostigma viride
rotenone 50% inhibition at 0.1 mM Mesostigma viride

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
glycolate
-
Mesostigma viride
9.3
-
L-lactate
-
Mesostigma viride

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-2 -hydroxyisocaproate + O2 Mesostigma viride 48% of the activity compared to glycolate 2-oxoisocaproate + H2O2
-
?
DL-2-hydroxybutyrate + O2 Mesostigma viride 68% of the activity compared to glycolate 2-oxobutyrate + H2O2
-
?
DL-2-hydroxyvalerate + O2 Mesostigma viride 39% of the activity compared to glycolate 2-oxovalerate + H2O2
-
?
glycolate + O2 Mesostigma viride
-
glyoxylate + H2O2
-
?
glyoxylate + O2 Mesostigma viride 26% of the activity compared to glycolate ? + H2O2
-
?
homoserine + O2 Mesostigma viride traces of activity ? + H2O2
-
?
L-lactate + O2 Mesostigma viride 28% of the activity compared to glycolate pyruvate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Mesostigma viride
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Mesostigma viride
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-2 -hydroxyisocaproate + O2 48% of the activity compared to glycolate Mesostigma viride 2-oxoisocaproate + H2O2
-
?
DL-2-hydroxybutyrate + O2 68% of the activity compared to glycolate Mesostigma viride 2-oxobutyrate + H2O2
-
?
DL-2-hydroxyvalerate + O2 39% of the activity compared to glycolate Mesostigma viride 2-oxovalerate + H2O2
-
?
glycolate + O2
-
Mesostigma viride glyoxylate + H2O2
-
?
glyoxylate + O2 26% of the activity compared to glycolate Mesostigma viride ? + H2O2
-
?
homoserine + O2 traces of activity Mesostigma viride ? + H2O2
-
?
L-lactate + O2 28% of the activity compared to glycolate Mesostigma viride pyruvate + H2O2
-
?

Synonyms

Synonyms Comment Organism
2-hydroxy acid oxidase 3 isoenzymes: HAOX1, HAOX2, HAOX3 Mesostigma viride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Mesostigma viride

pH Range

pH Minimum pH Maximum Comment Organism
8.3 9.5 80% of the maximum activity within Mesostigma viride

Cofactor

Cofactor Comment Organism Structure
FMN most effective electron acceptor Mesostigma viride