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Literature summary for 1.1.3.13 extracted from

  • Kjellander, M.; Goetz, K.; Liljeruhm, J.; Boman, M.; Johansson, G.
    Steady-state generation of hydrogen peroxide: kinetics and stability of alcohol oxidase immobilized on nanoporous alumina (2013), Biotechnol. Lett., 35, 585-590.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information the alcohol oxidase is immobilized on nanoporous aluminium oxide membranes by silanization and activation by carbonyldiimidazole to create a flow-through enzyme reactor, method optimization, overview. The immobilized alcohol oxidase shows high productivity in hydrogen peroxide generation from methanol, and retains about 58% of initial activity after 3 weeks of storage and repeated use with pH 6.5 and at room temperature. Construction of a bienzymatic modular reactor with horseradish peroxidase and alcohol oxidase, overview Komagataella pastoris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, overview Komagataella pastoris
0.47
-
methanol pH 6.5, 22°C, immobilized enzyme Komagataella pastoris
0.75
-
ethanol pH 6.5, 22°C, immobilized enzyme Komagataella pastoris
1.23
-
n-Propanol pH 6.5, 22°C, immobilized enzyme Komagataella pastoris
1.59
-
formaldehyde pH 6.5, 22°C, immobilized enzyme Komagataella pastoris
2.79
-
n-butanol pH 6.5, 22°C, immobilized enzyme Komagataella pastoris
8 9 ethylene glycol pH 6.5, 22°C, immobilized enzyme Komagataella pastoris
261
-
2-propanol pH 6.5, 22°C, immobilized enzyme Komagataella pastoris

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
4 * 80000, SDS-PAGE Komagataella pastoris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + O2 Komagataella pastoris
-
formaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Komagataella pastoris
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-propanol + O2
-
Komagataella pastoris 2-oxopropane + H2O2
-
?
ethanol + O2
-
Komagataella pastoris acetaldehyde + H2O2
-
?
ethylene glycol + O2
-
Komagataella pastoris ? + H2O2
-
?
formaldehyde + O2
-
Komagataella pastoris formic acid + H2O2
-
?
methanol + O2
-
Komagataella pastoris formaldehyde + H2O2
-
?
additional information substrate specificity, overview. No activity with isobutanol, benzyl alcohol, and cyclohexanol Komagataella pastoris ?
-
?
n-butanol + O2
-
Komagataella pastoris butanal + H2O2
-
?
n-propanol + O2
-
Komagataella pastoris propanal + H2O2
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 80000, SDS-PAGE Komagataella pastoris

Synonyms

Synonyms Comment Organism
AOX
-
Komagataella pastoris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Komagataella pastoris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Komagataella pastoris

Cofactor

Cofactor Comment Organism Structure
FAD
-
Komagataella pastoris