General Stability | Organism |
---|---|
the enzyme has increased stability in phosphate-citrate buffer and low stability in Tris-HCl and phosphate buffers. Its stability is increased 10fold in the presence of sucrose, mannitol, polyethylene glycol, polyvinyl alcohol, polyvinylpyrrolidone, and EDTA. Sucrose at 50% concentration (w/v) enhances stability of the enzyme 10fold at 50°C | Pichia putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | complete inhibition at molar excess of reagents in relation to alcohol oxidase 20:1 | Pichia putida | |
p-chloromercuribenzoate | complete inhibition at molar excess of reagents in relation to alcohol oxidase 20:1 | Pichia putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
8 * 74000, SDS-PAGE | Pichia putida |
580000 | - |
analytical ultracentrifugation | Pichia putida |
590000 | - |
gel filtration | Pichia putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pichia putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-cellulose column chromatography, Sepharose 2B column chromatography, and Sephadex G-25 gel filtration | Pichia putida |
Storage Stability | Organism |
---|---|
-20 to -80°C, 0.02 M phosphate-citrate buffer, pH 8-9, in presence of 30-50% (w/v) sucrose, 4 years, no loss of activity | Pichia putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + O2 | 92% activity compared to methanol | Pichia putida | acetaldehyde + H2O2 | - |
? | |
formaldehyde + O2 | 35% activity compared to methanol | Pichia putida | formate + H2O2 | - |
? | |
methanol + O2 | 100% activity | Pichia putida | formaldehyde + H2O2 | - |
? | |
n-butanol + O2 | 40% activity compared to methanol | Pichia putida | n-butanal + H2O2 | - |
? | |
n-pentanol + O2 | 3% activity compared to methanol | Pichia putida | n-pentanal + H2O2 | - |
? | |
n-propanol + O2 | 75% activity compared to methanol | Pichia putida | n-propanal + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooctamer | 8 * 74000, SDS-PAGE | Pichia putida |
Synonyms | Comment | Organism |
---|---|---|
alcohol:dioxygen-oxidoreductase | - |
Pichia putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | 10% loss of activity at 20°C after 24 h, complete loss of activity after 10 min at 60°C | Pichia putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Pichia putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | each subunit includes one molecule of FAD | Pichia putida |