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Literature summary for 1.1.3.10 extracted from
- Characterization of a bacterial pyranose 2-oxidase from Arthrobactersiccitolerans (2016), J. Mol. Catal. B, 133, s34-S43.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Star (DE3) cells | Pseudarthrobacter siccitolerans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
1,4-benzoquinone | with D-glucose as cosubstrate, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans |  |
| 1.1 | - |
D-glucose | with O2 as electron acceptor, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
| 2.1 | - |
D-glucose | with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
| 14 | - |
L-arabinose | with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
| 73 | - |
D-xylose | with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
| 101 | - |
D-galactose | with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
| 180 | - |
D-ribose | with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58500 | - |
- |
Pseudarthrobacter siccitolerans |
| 63600 | - |
gel filtration | Pseudarthrobacter siccitolerans |
| 66000 | - |
1 * 66000, SDS-PAGE | Pseudarthrobacter siccitolerans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + O2 | Pseudarthrobacter siccitolerans | - |
2-dehydro-D-glucose + H2O2 | - |
? | |
| D-glucose + O2 | Pseudarthrobacter siccitolerans 4J27 | - |
2-dehydro-D-glucose + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudarthrobacter siccitolerans | - |
- |
- |
| Pseudarthrobacter siccitolerans 4J27 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography | Pseudarthrobacter siccitolerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose + 1,4-benzoquinone | - |
Pseudarthrobacter siccitolerans | 2-dehydro-D-galactose + 1,4-hydroquinone | - |
? | |
| D-galactose + 1,4-benzoquinone | - |
Pseudarthrobacter siccitolerans 4J27 | 2-dehydro-D-galactose + 1,4-hydroquinone | - |
? | |
| D-galactose + O2 | - |
Pseudarthrobacter siccitolerans | 2-dehydro-D-galactose + H2O2 | - |
? | |
| D-galactose + O2 | - |
Pseudarthrobacter siccitolerans 4J27 | 2-dehydro-D-galactose + H2O2 | - |
? | |
| D-glucose + 1,4-benzoquinone | - |
Pseudarthrobacter siccitolerans | 2-dehydro-D-glucose + 1,4-hydroquinone | - |
? | |
| D-glucose + O2 | - |
Pseudarthrobacter siccitolerans | 2-dehydro-D-glucose + H2O2 | - |
? | |
| D-glucose + O2 | D-glucose is the best substrate | Pseudarthrobacter siccitolerans | 2-dehydro-D-glucose + H2O2 | - |
? | |
| D-glucose + O2 | - |
Pseudarthrobacter siccitolerans 4J27 | 2-dehydro-D-glucose + H2O2 | - |
? | |
| D-glucose + O2 | D-glucose is the best substrate | Pseudarthrobacter siccitolerans 4J27 | 2-dehydro-D-glucose + H2O2 | - |
? | |
| D-ribose + 1,4-benzoquinone | - |
Pseudarthrobacter siccitolerans | 2-dehydro-D-ribose + 1,4-hydroquinone | - |
? | |
| D-ribose + O2 | - |
Pseudarthrobacter siccitolerans | 2-dehydro-D-ribose + H2O2 | - |
? | |
| D-xylose + 1,4-benzoquinone | - |
Pseudarthrobacter siccitolerans | 2-dehydro-D-xylose + 1,4-hydroquinone | - |
? | |
| D-xylose + O2 | - |
Pseudarthrobacter siccitolerans | 2-dehydro-D-xylose + H2O2 | - |
? | |
| L-arabinose + 1,4-benzoquinone | - |
Pseudarthrobacter siccitolerans | 2-dehydro-L-arabinose + 1,4-hydroquinone | - |
? | |
| L-arabinose + O2 | - |
Pseudarthrobacter siccitolerans | 2-dehydro-L-arabinose + H2O2 | - |
? | |
| additional information | no activity in the presence of D-mannose, D-fructose, maltose, trehalose, lactose and D-melibiose | Pseudarthrobacter siccitolerans | ? | - |
? | |
| additional information | no activity in the presence of D-mannose, D-fructose, maltose, trehalose, lactose and D-melibiose | Pseudarthrobacter siccitolerans 4J27 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, SDS-PAGE | Pseudarthrobacter siccitolerans |
| monomer | 1 * 58500, calculated from amino acid sequence | Pseudarthrobacter siccitolerans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| P2Ox | - |
Pseudarthrobacter siccitolerans |
| pyranose 2-Oxidase | - |
Pseudarthrobacter siccitolerans |
| pyranose:oxygen 2-oxidoreductase | - |
Pseudarthrobacter siccitolerans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Pseudarthrobacter siccitolerans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 34 | 40 | more than 50% activity between 34 and 40°C | Pseudarthrobacter siccitolerans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 43 | the enzyme shows a melting temperature of 43°C and a half-life at 40°C of 25 min | Pseudarthrobacter siccitolerans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.153 | - |
D-glucose | with O2 as electron acceptor, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
| 0.19 | - |
L-arabinose | with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
| 0.38 | - |
D-ribose | with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
| 0.39 | - |
1,4-benzoquinone | with D-glucose as cosubstrate, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
| 0.48 | - |
D-glucose | with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
| 0.57 | - |
D-galactose | with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
| 0.91 | - |
D-xylose | with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C | Pseudarthrobacter siccitolerans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Pseudarthrobacter siccitolerans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | at pH 8.0, the enzyme still retains 45% of its maximal activity observed at pH 6.5 | Pseudarthrobacter siccitolerans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudarthrobacter siccitolerans | |
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