Literature summary for 1.1.3.10 extracted from

Halada, P.; Brugger, D.; Volc, J.; Peterbauer, C.K.; Leitner, C.; Haltrich, D.
Oxidation of Phe454 in the gating segment inactivates Trametes multicolor pyranose oxidase during substrate turnover (2016), PLoS ONE, 11, e0148108.

Search for more literature for 1.1.3.10

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 Star DE3 cells	Trametes ochracea

Protein Variants

Protein Variants	Comment	Organism
F454A	the mutant shows about 40fold reduced catalytic efficiency compared to the wild type enzyme	Trametes ochracea
F454Y	the mutant shows about 3.5fold reduced catalytic efficiency compared to the wild type enzyme	Trametes ochracea

Inhibitors

Inhibitors	Comment	Organism	Structure
H2O2	oxidation of Phe454, which is located at the flexible active-site loop of the enzyme, is the first and main step in the inactivation of the enzyme by hydrogen peroxide	Trametes ochracea

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.712	-	D-glucose	mutant enzyme F454A, at pH 6.5 and 30°C	Trametes ochracea
0.851	-	D-glucose	wild type enzyme, at pH 6.5 and 30°C	Trametes ochracea
0.948	-	D-glucose	wild type enzyme F454Y, at pH 6.5 and 30°C	Trametes ochracea

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
270000	-	gel filtration	Trametes ochracea

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucose + O2	Trametes ochracea	-	2-dehydro-D-glucose + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Trametes ochracea	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
IMAC Ni-charged resin column chromatography	Trametes ochracea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose + O2	-	Trametes ochracea	2-dehydro-D-glucose + H2O2	-	?

Subunits

Subunits	Comment	Organism
homotetramer	-	Trametes ochracea

Synonyms

Synonyms	Comment	Organism
POX	-	Trametes ochracea
pyranose:oxygen 2-oxidoreductase	-	Trametes ochracea

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.479	-	D-glucose	mutant enzyme F454A, at pH 6.5 and 30°C	Trametes ochracea
16.3	-	D-glucose	wild type enzyme F454Y, at pH 6.5 and 30°C	Trametes ochracea
49	-	D-glucose	wild type enzyme, at pH 6.5 and 30°C	Trametes ochracea

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Trametes ochracea

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.47	-	D-glucose	mutant enzyme F454A, at pH 6.5 and 30°C	Trametes ochracea
17.2	-	D-glucose	wild type enzyme F454Y, at pH 6.5 and 30°C	Trametes ochracea
57.6	-	D-glucose	wild type enzyme, at pH 6.5 and 30°C	Trametes ochracea

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Release 2023.1 (January 2023)