Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Aspergillus nidulans |
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
2-methyl-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
0.15 | - |
1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
0.32 | - |
1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
0.39 | - |
2,6-dichloroindophenol | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
0.4 | - |
2,6-dimethyl-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
0.42 | - |
2-methoxy-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
0.48 | - |
2-chloro-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
0.67 | - |
ferricenium hexafluorophosphate | pH 8.0, 30°C, recombinant enzyme | Aspergillus nidulans | |
1.12 | - |
ferricenium hexafluorophosphate | pH 8.0, 30°C, recombinant enzyme | Aspergillus oryzae | |
1.77 | - |
D-glucose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
2.86 | - |
D-glucose | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
9.42 | - |
tetrafluoro-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
13 | - |
D-galactose | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
18.1 | - |
D-xylose | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
32.6 | - |
L-sorbose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
66.9 | - |
D-xylose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
176 | - |
L-sorbose | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
192 | - |
D-galactose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
2558 | - |
D-fructose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Aspergillus nidulans |
65000 | - |
x * 65000, SDS-PAGE | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | Aspergillus nidulans | - |
2-dehydro-D-glucose + H2O2 | - |
? | |
D-glucose + O2 | Aspergillus oryzae | - |
2-dehydro-D-glucose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
- |
- |
Aspergillus oryzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 232fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Aspergillus oryzae |
recombinant His-tagged enzyme 6.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Aspergillus nidulans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.58 | - |
purified recombinant His-tagged enzyme, pH 6.5, 30°C | Aspergillus oryzae |
1.16 | - |
purified recombinant His-tagged enzyme, pH 6.5, 30°C | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + O2 | - |
Aspergillus nidulans | 2-dehydro-D-fructose + H2O2 | - |
? | |
D-galactose + O2 | - |
Aspergillus nidulans | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-galactose + O2 | - |
Aspergillus oryzae | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Aspergillus nidulans | ? | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Aspergillus oryzae | ? | - |
? | |
D-glucose + 2,6-dichloroindophenol | - |
Aspergillus nidulans | ? | - |
? | |
D-glucose + 2,6-dimethyl-1,4-benzoquinone | - |
Aspergillus nidulans | ? | - |
? | |
D-glucose + 2-chloro-1,4-benzoquinone | - |
Aspergillus nidulans | ? | - |
? | |
D-glucose + 2-methoxy-1,4-benzoquinone | - |
Aspergillus nidulans | ? | - |
? | |
D-glucose + 2-methyl-1,4-benzoquinone | - |
Aspergillus nidulans | ? | - |
? | |
D-glucose + ferricenium hexafluorophosphate | - |
Aspergillus nidulans | ? | - |
? | |
D-glucose + ferricenium hexafluorophosphate | - |
Aspergillus oryzae | ? | - |
? | |
D-glucose + O2 | - |
Aspergillus nidulans | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-glucose + O2 | - |
Aspergillus oryzae | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-glucose + tetrafluoro-1,4-benzoquinone | - |
Aspergillus nidulans | ? | - |
? | |
D-xylose + O2 | - |
Aspergillus nidulans | 2-dehydro-D-xylose + H2O2 | - |
? | |
D-xylose + O2 | - |
Aspergillus oryzae | 2-dehydro-D-xylose + H2O2 | - |
? | |
L-sorbose + O2 | - |
Aspergillus nidulans | 5-dehydro-D-fructose + H2O2 | - |
? | |
L-sorbose + O2 | - |
Aspergillus oryzae | 5-dehydro-D-fructose + H2O2 | - |
? | |
additional information | no activity with D-fructose, mannose and lactose as substrates. Peroxidase-coupled assay using [2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid)] as the chromogen | Aspergillus oryzae | ? | - |
? | |
additional information | no activity with mannose and lactose as substrates. The enzyme catalyzes the two-electron reduction of 1,4-benzoquinone, several substituted benzoquinones and 2,6-dichloroindophenol. Some of these quinone electron acceptors are better substrates for pyranose oxidase than oxygen. Peroxidase-coupled assay using [2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid)] as the chromogen | Aspergillus nidulans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Aspergillus nidulans |
? | x * 65000, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
glucose 2-oxidase | - |
Aspergillus nidulans |
glucose 2-oxidase | - |
Aspergillus oryzae |
POX | - |
Aspergillus nidulans |
POX | - |
Aspergillus oryzae |
pyranose 2-Oxidase | - |
Aspergillus nidulans |
pyranose 2-Oxidase | - |
Aspergillus oryzae |
pyranose/oxygen 2-oxidoreductase | - |
Aspergillus nidulans |
pyranose/oxygen 2-oxidoreductase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus nidulans |
30 | - |
assay at | Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
D-galactose | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
0.27 | - |
D-xylose | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
0.4 | - |
2,6-dimethyl-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
1.39 | - |
D-xylose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
1.48 | - |
D-glucose | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
1.59 | - |
L-sorbose | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
1.7 | - |
ferricenium hexafluorophosphate | pH 8.0, 30°C, recombinant enzyme | Aspergillus oryzae | |
1.95 | - |
D-galactose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
3.04 | - |
1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
5.55 | - |
D-fructose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
9.66 | - |
L-sorbose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
10.1 | - |
2-methyl-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
13.1 | - |
2,6-dichloroindophenol | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
16.3 | - |
2-chloro-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
35.44 | - |
D-glucose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
47.2 | - |
2-methoxy-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
129 | - |
tetrafluoro-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
158 | - |
ferricenium hexafluorophosphate | pH 8.0, 30°C, recombinant enzyme | Aspergillus nidulans | |
186 | - |
1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Aspergillus nidulans |
6.5 | - |
assay at | Aspergillus oryzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavoprotein | Aspergillus nidulans | |
flavin | flavoprotein | Aspergillus oryzae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
D-galactose | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
0.009 | - |
L-sorbose | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
0.015 | - |
D-xylose | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
0.02 | - |
D-xylose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
0.1 | - |
D-galactose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
0.1 | - |
2,6-dimethyl-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
0.3 | - |
L-sorbose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
0.52 | - |
D-glucose | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
1.5 | - |
ferricenium hexafluorophosphate | pH 8.0, 30°C, recombinant enzyme | Aspergillus oryzae | |
9.4 | - |
1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus oryzae | |
13.7 | - |
tetrafluoro-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
20.02 | - |
D-glucose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
22 | - |
D-fructose | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
33.6 | - |
2,6-dichloroindophenol | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
34 | - |
2-chloro-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
91.8 | - |
2-methyl-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
112 | - |
2-methoxy-1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans | |
235 | - |
ferricenium hexafluorophosphate | pH 8.0, 30°C, recombinant enzyme | Aspergillus nidulans | |
1240 | - |
1,4-benzoquinone | pH 6.5, 30°C, recombinant enzyme | Aspergillus nidulans |