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Literature summary for 1.1.3.10 extracted from

  • Pisanelli, I.; Wuehrer, P.; Reyes-Dominguez, Y.; Spadiut, O.; Haltrich, D.; Peterbauer, C.
    Heterologous expression and biochemical characterization of novel pyranose 2-oxidases from the ascomycetes Aspergillus nidulans and Aspergillus oryzae (2012), Appl. Microbiol. Biotechnol., 93, 1157-1166.
    View publication on PubMed
Search for more literature for 1.1.3.10

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Aspergillus nidulans
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 2-methyl-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
0.15
-
 1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
0.32
-
 1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
0.39
-
 2,6-dichloroindophenol pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
0.4
-
 2,6-dimethyl-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
0.42
-
 2-methoxy-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
0.48
-
 2-chloro-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
0.67
-
 ferricenium hexafluorophosphate pH 8.0, 30°C, recombinant enzyme Aspergillus nidulans
1.12
-
 ferricenium hexafluorophosphate pH 8.0, 30°C, recombinant enzyme Aspergillus oryzae
1.77
-
 D-glucose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
2.86
-
 D-glucose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
9.42
-
 tetrafluoro-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
13
-
 D-galactose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
18.1
-
 D-xylose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
32.6
-
 L-sorbose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
66.9
-
 D-xylose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
176
-
 L-sorbose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
192
-
 D-galactose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
2558
-
 D-fructose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Aspergillus nidulans
65000
-
 x * 65000, SDS-PAGE Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + O2 Aspergillus nidulans
-
 2-dehydro-D-glucose + H2O2
-
 ?
D-glucose + O2 Aspergillus oryzae
-
 2-dehydro-D-glucose + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 232fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Aspergillus oryzae
recombinant His-tagged enzyme 6.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Aspergillus nidulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.58
-
 purified recombinant His-tagged enzyme, pH 6.5, 30°C Aspergillus oryzae
1.16
-
 purified recombinant His-tagged enzyme, pH 6.5, 30°C Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + O2
-
 Aspergillus nidulans 2-dehydro-D-fructose + H2O2
-
 ?
D-galactose + O2
-
 Aspergillus nidulans 2-dehydro-D-galactose + H2O2
-
 ?
D-galactose + O2
-
 Aspergillus oryzae 2-dehydro-D-galactose + H2O2
-
 ?
D-glucose + 1,4-benzoquinone
-
 Aspergillus nidulans ?
-
 ?
D-glucose + 1,4-benzoquinone
-
 Aspergillus oryzae ?
-
 ?
D-glucose + 2,6-dichloroindophenol
-
 Aspergillus nidulans ?
-
 ?
D-glucose + 2,6-dimethyl-1,4-benzoquinone
-
 Aspergillus nidulans ?
-
 ?
D-glucose + 2-chloro-1,4-benzoquinone
-
 Aspergillus nidulans ?
-
 ?
D-glucose + 2-methoxy-1,4-benzoquinone
-
 Aspergillus nidulans ?
-
 ?
D-glucose + 2-methyl-1,4-benzoquinone
-
 Aspergillus nidulans ?
-
 ?
D-glucose + ferricenium hexafluorophosphate
-
 Aspergillus nidulans ?
-
 ?
D-glucose + ferricenium hexafluorophosphate
-
 Aspergillus oryzae ?
-
 ?
D-glucose + O2
-
 Aspergillus nidulans 2-dehydro-D-glucose + H2O2
-
 ?
D-glucose + O2
-
 Aspergillus oryzae 2-dehydro-D-glucose + H2O2
-
 ?
D-glucose + tetrafluoro-1,4-benzoquinone
-
 Aspergillus nidulans ?
-
 ?
D-xylose + O2
-
 Aspergillus nidulans 2-dehydro-D-xylose + H2O2
-
 ?
D-xylose + O2
-
 Aspergillus oryzae 2-dehydro-D-xylose + H2O2
-
 ?
L-sorbose + O2
-
 Aspergillus nidulans 5-dehydro-D-fructose + H2O2
-
 ?
L-sorbose + O2
-
 Aspergillus oryzae 5-dehydro-D-fructose + H2O2
-
 ?
additional information no activity with D-fructose, mannose and lactose as substrates. Peroxidase-coupled assay using [2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid)] as the chromogen Aspergillus oryzae ?
-
 ?
additional information no activity with mannose and lactose as substrates. The enzyme catalyzes the two-electron reduction of 1,4-benzoquinone, several substituted benzoquinones and 2,6-dichloroindophenol. Some of these quinone electron acceptors are better substrates for pyranose oxidase than oxygen. Peroxidase-coupled assay using [2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid)] as the chromogen Aspergillus nidulans ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Aspergillus nidulans
? x * 65000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
glucose 2-oxidase
-
 Aspergillus nidulans
glucose 2-oxidase
-
 Aspergillus oryzae
POX
-
 Aspergillus nidulans
POX
-
 Aspergillus oryzae
pyranose 2-Oxidase
-
 Aspergillus nidulans
pyranose 2-Oxidase
-
 Aspergillus oryzae
pyranose/oxygen 2-oxidoreductase
-
 Aspergillus nidulans
pyranose/oxygen 2-oxidoreductase
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Aspergillus nidulans
30
-
 assay at Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 D-galactose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
0.27
-
 D-xylose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
0.4
-
 2,6-dimethyl-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.39
-
 D-xylose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.48
-
 D-glucose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.59
-
 L-sorbose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.7
-
 ferricenium hexafluorophosphate pH 8.0, 30°C, recombinant enzyme Aspergillus oryzae
1.95
-
 D-galactose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
3.04
-
 1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
5.55
-
 D-fructose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
9.66
-
 L-sorbose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
10.1
-
 2-methyl-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
13.1
-
 2,6-dichloroindophenol pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
16.3
-
 2-chloro-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
35.44
-
 D-glucose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
47.2
-
 2-methoxy-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
129
-
 tetrafluoro-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
158
-
 ferricenium hexafluorophosphate pH 8.0, 30°C, recombinant enzyme Aspergillus nidulans
186
-
 1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Aspergillus nidulans
6.5
-
 assay at Aspergillus oryzae

Cofactor

Cofactor Comment Organism Structure
flavin flavoprotein Aspergillus nidulans
flavin flavoprotein Aspergillus oryzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.002
-
 D-galactose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
0.009
-
 L-sorbose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
0.015
-
 D-xylose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
0.02
-
 D-xylose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
0.1
-
 D-galactose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
0.1
-
 2,6-dimethyl-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
0.3
-
 L-sorbose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
0.52
-
 D-glucose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.5
-
 ferricenium hexafluorophosphate pH 8.0, 30°C, recombinant enzyme Aspergillus oryzae
9.4
-
 1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
13.7
-
 tetrafluoro-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
20.02
-
 D-glucose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
22
-
 D-fructose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
33.6
-
 2,6-dichloroindophenol pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
34
-
 2-chloro-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
91.8
-
 2-methyl-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
112
-
 2-methoxy-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
235
-
 ferricenium hexafluorophosphate pH 8.0, 30°C, recombinant enzyme Aspergillus nidulans
1240
-
 1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans