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Literature summary for 1.1.3.10 extracted from

  • Spadiut, O.; Radakovits, K.; Pisanelli, I.; Salaheddin, C.; Yamabhai, M.; Tan, T.C.; Divne, C.; Haltrich, D.
    A thermostable triple mutant of pyranose 2-oxidase from Trametes multicolor with improved properties for biotechnological applications (2009), Biotechnol. J., 4, 525-534.
    View publication on PubMed
Search for more literature for 1.1.3.10

Protein Variants

Protein Variants Comment Organism
T169G/E542K/V546C mutant, analysis of kinetic parameters and thermal stability Trametes ochracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.093
-
 D-galactose T169G/E542K/V546C, mutant, 30°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
0.19
-
 D-galactose T169G/E542K/V546C, mutant, 50°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
0.22
-
 D-glucose T169G/E542K/V546C, mutant, 30°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
0.23
-
 D-galactose wild-type, mutant, 50°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
0.25
-
 D-galactose wild-type, mutant, 30°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
0.31
-
 D-glucose T169G/E542K/V546C, mutant, 50°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
0.4
-
 D-glucose wild-type, mutant, 30°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
0.64
-
 D-glucose T169G/E542K/V546C, mutant, 30°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
0.76
-
 D-glucose wild-type, mutant, 30°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
0.78
-
 D-glucose wild-type, mutant, 50°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.15
-
 D-glucose T169G/E542K/V546C, mutant, 50°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.18
-
 D-glucose wild-type, mutant, 50°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.66
-
 D-galactose T169G/E542K/V546C, mutant, 30°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
2.76
-
 D-galactose T169G/E542K/V546C, mutant, 50°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
7.94
-
 D-galactose wild-type, mutant, 30°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
14.6
-
 D-galactose wild-type, mutant, 50°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea

Organism

Organism UniProt Comment Textmining
Trametes ochracea Q7ZA32
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 kinetic parameters measured at batch conversion experiments of wild-type pyranose oxidase and mutant T169G/E542K/V546C Trametes ochracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + 1,4-benzoquinone
-
 Trametes ochracea 2-dehydro-D-galactose + 1,4-hydroquinone
-
 ?
D-galactose + O2
-
 Trametes ochracea 2-dehydro-D-galactose + H2O2
-
 ?
D-glucose + 1,4-benzoquinone
-
 Trametes ochracea 2-dehydro-D-glucose + 1,4-hydroquinone
-
 ?
D-glucose + O2
-
 Trametes ochracea 2-dehydro-D-glucose + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
P2Ox
-
 Trametes ochracea
pyranose oxidase
-
 Trametes ochracea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 T169G/E542K/V546C mutant, half life is increased 76fold compared to wild-type Trametes ochracea
70
-
 T169G/E542K/V546C mutant, half life is increased 350fold compared to wild-type Trametes ochracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.072
-
 D-glucose T169G/E542K/V546C, mutant, 30°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
0.27
-
 D-galactose T169G/E542K/V546C, mutant, 30°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
0.35
-
 D-glucose T169G/E542K/V546C, mutant, 50°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
0.74
-
 D-galactose T169G/E542K/V546C, mutant, 50°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
2.1
-
 D-galactose wild-type, mutant, 30°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
5.51
-
 D-galactose wild-type, mutant, 50°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
6.61
-
 D-galactose wild-type, mutant, 30°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
14.6
-
 D-galactose wild-type, mutant, 50°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
21.16
-
 D-glucose T169G/E542K/V546C, mutant, 30°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
34
-
 D-glucose wild-type, mutant, 30°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
58.9
-
 D-glucose wild-type, mutant, 50°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
79.3
-
 D-glucose T169G/E542K/V546C, mutant, 50°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
171
-
 D-galactose T169G/E542K/V546C, mutant, 50°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
349
-
 D-glucose wild-type, mutant, 30°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
615
-
 D-glucose wild-type, mutant, 50°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea