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Literature summary for 1.1.3.10 extracted from

  • Rungsrisuriyachai, K.; Gadda, G.
    A pH switch affects the steady-state kinetic mechanism of pyranose 2-oxidase from Trametes ochracea (2009), Arch. Biochem. Biophys., 483, 10-15.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of pyranose 2-oxidase in Escherichia coli strain Rosetta(DE3)pLysS Trametes ochracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
O2 pH 5.5, substrate oxygen limited (D-glucose excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C Trametes ochracea
0.04
-
O2 pH 8.5, substrate D-glucose limited (oxygen excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C Trametes ochracea
0.1
-
O2 pH 6.5, substrate oxygen limited (D-glucose excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C Trametes ochracea
0.1
-
O2 pH 7.5, substrate oxygen limited (D-glucose excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C Trametes ochracea
0.2
-
O2 pH 7.5, substrate D-glucose limited (oxygen excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C Trametes ochracea
0.3
-
O2 pH 5.5, substrate D-glucose limited (oxygen excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C Trametes ochracea
0.5
-
O2 pH 8.5, substrate oxygen limited (D-glucose excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C Trametes ochracea
0.9
-
O2 pH 6.5, substrate D-glucose limited (oxygen excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C Trametes ochracea

Organism

Organism UniProt Comment Textmining
Trametes ochracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
stored, wet cell paste is treated with lysozyme, PMSF, EDTA buffer. Following steps are RNAse and DNAse treatment, sonication, dialyse, DEAE Sepharose Fast Flow column, dialyse, Phenyl Sepharose Fast Flow column, concentration by ammonium sulfate saturation, final dialyse. Stored at 20°C Trametes ochracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + O2
-
Trametes ochracea 2-dehydro-D-glucose + H2O2
-
?

Synonyms

Synonyms Comment Organism
glucose 2-oxidase
-
Trametes ochracea
pyranose 2-Oxidase
-
Trametes ochracea
pyranose:oxygen 2-oxidoreductase
-
Trametes ochracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.9
-
O2 pH 5.5, substrate D-glucose, measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C Trametes ochracea
6.5
-
O2 pH 7.5, substrate D-glucose, measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C Trametes ochracea
6.8
-
O2 pH 6.5, substrate D-glucose, measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C Trametes ochracea
7.5
-
O2 pH 8.5, substrate D-glucose, measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C Trametes ochracea

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 substrate D-glucose, measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C, varying concentrations of both D-glucose and oxygen. Ping-pong kinetic mechanism at pH values below 7.0 and an ordered mechanism at pH above 7.0 Trametes ochracea