Cloned (Comment) | Organism |
---|---|
recombinant expression of pyranose 2-oxidase in Escherichia coli strain Rosetta(DE3)pLysS | Trametes ochracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
O2 | pH 5.5, substrate oxygen limited (D-glucose excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C | Trametes ochracea | |
0.04 | - |
O2 | pH 8.5, substrate D-glucose limited (oxygen excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C | Trametes ochracea | |
0.1 | - |
O2 | pH 6.5, substrate oxygen limited (D-glucose excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C | Trametes ochracea | |
0.1 | - |
O2 | pH 7.5, substrate oxygen limited (D-glucose excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C | Trametes ochracea | |
0.2 | - |
O2 | pH 7.5, substrate D-glucose limited (oxygen excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C | Trametes ochracea | |
0.3 | - |
O2 | pH 5.5, substrate D-glucose limited (oxygen excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C | Trametes ochracea | |
0.5 | - |
O2 | pH 8.5, substrate oxygen limited (D-glucose excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C | Trametes ochracea | |
0.9 | - |
O2 | pH 6.5, substrate D-glucose limited (oxygen excess), measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C | Trametes ochracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
stored, wet cell paste is treated with lysozyme, PMSF, EDTA buffer. Following steps are RNAse and DNAse treatment, sonication, dialyse, DEAE Sepharose Fast Flow column, dialyse, Phenyl Sepharose Fast Flow column, concentration by ammonium sulfate saturation, final dialyse. Stored at 20°C | Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | - |
Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucose 2-oxidase | - |
Trametes ochracea |
pyranose 2-Oxidase | - |
Trametes ochracea |
pyranose:oxygen 2-oxidoreductase | - |
Trametes ochracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
O2 | pH 5.5, substrate D-glucose, measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C | Trametes ochracea | |
6.5 | - |
O2 | pH 7.5, substrate D-glucose, measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C | Trametes ochracea | |
6.8 | - |
O2 | pH 6.5, substrate D-glucose, measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C | Trametes ochracea | |
7.5 | - |
O2 | pH 8.5, substrate D-glucose, measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C | Trametes ochracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | substrate D-glucose, measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C, varying concentrations of both D-glucose and oxygen. Ping-pong kinetic mechanism at pH values below 7.0 and an ordered mechanism at pH above 7.0 | Trametes ochracea |