Application | Comment | Organism |
---|---|---|
biotechnology | studies on biosensors and biofuel cell anodes | Trametes ochracea |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-DE3, recombinant protein, plasmids pHL2e and pCL22 encoding the E542K mutant used | Trametes ochracea |
Protein Variants | Comment | Organism |
---|---|---|
E542K | inserted into plasmid pCL22 | Trametes ochracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | D-mannose, glucosamine, beta-lactose, sucrose and D-maltose lack response signal, higher maltooligosaccharides good substrates for pyranose 2-oxidase-based biosensor | Trametes ochracea | |
0.32 | - |
D-glucose | wild-type protein, 0.1 M ethanolamine buffer at pH 10.5 | Trametes ochracea | |
0.35 | - |
D-glucose | recombinant protein with a hexa-histidine tag, 0.1 M ethanolamine buffer at pH 10.5 | Trametes ochracea | |
0.71 | - |
D-cellobiose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
1.51 | - |
D-glucose | E542K with a hexa-histidine tag, 0.1 M ethanolamine buffer at pH 10.5 | Trametes ochracea | |
1.55 | - |
L-arabinose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
2.72 | - |
D-glucose | recombinant protein, 0.1 M PBS buffer at pH 8 | Trametes ochracea | |
6.02 | - |
D-maltoheptaose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
6.14 | - |
D-maltopentaose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
6.21 | - |
D-xylose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
6.25 | - |
D-fucose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
6.61 | - |
D-maltotriose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
7.51 | - |
D-trehalose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
10.32 | - |
2-deoxy-D-galactose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
11.81 | - |
D-galactose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
11.85 | - |
2-deoxy-D-glucose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | Q7ZA32 | wild-type, recombinant protein and commercial preparation | - |
Purification (Comment) | Organism |
---|---|
wild-type and recombinant protein, gel purification | Trametes ochracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
pyranose 2-oxidase-based biosensor system, electrical wiring of different types of pyranose oxidase with an osmium redox polymer on graphite electrodes, optimization studies using glucose as substrate, biosensors with best characteristics in terms of linear range, detection limit and sensitivity used for analyzing selectivity for different sugar substrates | Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-galactose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
2-deoxy-D-glucose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
D-cellobiose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
D-fucose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
D-galactose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
D-glucose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-maltoheptaose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
D-maltopentaose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
D-maltotriose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
D-trehalose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
D-xylose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
L-arabinose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
under dry and wet storage conditions stable at 4°C, under dry storage conditions retainment of 100% of original activity after 2 days and of about 90% after one week, storage in working buffer solutions decreases activity by about 10% after one day and by more than 50% after one week | Trametes ochracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
activity assay at | Trametes ochracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | optimal buffer combination for the pyranose 2-oxidase systems determined | Trametes ochracea |