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Literature summary for 1.1.3.10 extracted from

  • Izumi, Y.; Furuya, Y.; Yamada, H.
    Purification and properties of pyranose oxidase from Basidiomycetous fungus No. 52 (1990), Agric. Biol. Chem., 54, 1393-1399.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
NaCl up to 8% Basidiomycota

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline weak inhibitor Basidiomycota
8-hydroxyquinoline weak inhibitor Basidiomycota
Ag+ strong inhibitor Basidiomycota
Cu2+ weak inhibitor Basidiomycota
ethanol 17% inhibition by 5% ethanol and 29% inhibition by 10% ethanol Basidiomycota
Hg2+ strong inhibitor Basidiomycota
NaCl slightly inhibits at concentrations higher than 8% Basidiomycota

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ stimulation Basidiomycota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
4 * 69000, SDS-PAGE Basidiomycota
300000
-
gel filtration Basidiomycota

Organism

Organism UniProt Comment Textmining
Basidiomycota
-
unidentified
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 0.7% carbohydrate Basidiomycota

Purification (Commentary)

Purification (Comment) Organism
using column chromatography on HPA-75, Phenyl-Sepharose CL-6B and cellulofine GCL-2000 superfine Basidiomycota

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.3
-
purified enzyme Basidiomycota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-deoxy-D-glucose + O2
-
Basidiomycota 2-keto-6-deoxy-D-glucose + H2O2
-
?
D-fucono-1,5-lactone + O2
-
Basidiomycota 2-keto-D-gluconate + D-araboascorbate + H2O2
-
?
D-gluconolactone + O2 activity about 10% that of D-glucose Basidiomycota ? + H2O2
-
?
D-glucose + O2 D-glucose is the preferred substrate Basidiomycota D-glucosone + H2O2
-
?
D-xylose + O2 activity about 10% that of D-glucose Basidiomycota D-xylosone + H2O2
-
?
L-sorbose + O2 activity about 10% that of D-glucose Basidiomycota 5-dehydro-D-fructose + H2O2
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 69000, SDS-PAGE Basidiomycota

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Basidiomycota

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
not stable above Basidiomycota
60
-
88% of its activity remains Basidiomycota
70
-
60% of its initial activity remains Basidiomycota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 8
-
Basidiomycota

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
67% of its initial activity remains, citrate buffer Basidiomycota
5.5 9 no loss of activity on incubation for 30 min at 50°C Basidiomycota
11
-
78% of its initial activity remains on incubation for 30 min at 50°C, glycine-NaOH buffer Basidiomycota

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound Basidiomycota