Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.2.8 extracted from

  • Wehrmann, M.; Klebensberger, J.
    Engineering thermal stability and solvent tolerance of the soluble quinoprotein PedE from Pseudomonas putida KT2440 with a heterologous whole-cell screening approach (2018), Microb. Biotechnol., 11, 399-408 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.2.8

Cloned(Commentary)

Cloned (Comment) Organism
C-terminally His-tagged wild-type and mutant enzyme forms are expressed in Escherichia coli BL21(DE3) Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
E408P mutant shows a 2.3fold increased stability upon incubation at 45°C for 1 h compared with the wild-type allele Pseudomonas putida
N410K 44% relative residual activities compared with 23% for the wild-type allele upon 1 h incubation at 45°C Pseudomonas putida
N410S about 30% relative residual activities compared with 23% for the wild-type allele upon 1 h incubation at 45°C Pseudomonas putida
N410T about 35% relative residual activities compared with 23% for the wild-type allele upon 1 h incubation at 45°C Pseudomonas putida
R91D 41% relative residual activities compared with 23% for the wild-type allele upon 1 h incubation at 45°C Pseudomonas putida
R91D/E408P mutant shows a 3.2fold stability upon incubation at 45°C for 1 h compared with the wild-type allele Pseudomonas putida
R91D/E408P/N410K mutant shows a 4.0fold increased stability upon incubation at 45°C for 1 h compared with the wild-type allele Pseudomonas putida
R91D/E408P/N410K the mutant exhibits a 7°C increase in thermal stability but also a twofold increase in residual activity upon incubation with up to 50% dimethyl sulfoxide, while showing no significant difference in enzymatic efficiency (kcat/KM) Pseudomonas putida
R91E about 35% relative residual activities compared with 23% for the wild-type allele upon 1 h incubation at 45°C Pseudomonas putida
R91Q about 30% relative residual activities compared with 23% for the wild-type allele upon 1 h incubation at 45°C Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 ethanol mutant enzyme R91D/E408P/N410K, pH and temperature not specified in the publication Pseudomonas putida
0.074
-
 ethanol wild-type enzyme, pH and temperature not specified in the publication Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88JH5
-
-

Purification (Commentary)

Purification (Comment) Organism
C-terminally His-tagged PQQ-ADHs are expressed in Escherichia coli BL21(DE3) Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylethanol + phenazine methosulfate converted with 76% of the activity determined with ethanol Pseudomonas putida phenylacetaldehyde + reduced phenazine methosulfate
-
 ?
acetaldehyde + phenazine methosulfate converted with 71% of the activity determined with ethanol Pseudomonas putida ? + reduced phenazine methosulfate
-
 ?
ethanol + phenazine methosulfate
-
 Pseudomonas putida acetaldehyde + reduced phenazine methosulfate
-
 ?

Synonyms

Synonyms Comment Organism
PedE
-
 Pseudomonas putida
PP_2674
-
 Pseudomonas putida
PQQ-ADH
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 relative residual activity upon 1 h incubation: about 20% (wild-type enzyme), 41% (mutant enzyme R19D), about 35% (mutant enzyme R191E), about 30% (mutant enzyme R191Q), 44% (mutant enzyme N410K), about 40% (mutant enzyme N410T), about 30% (mutant enzyme N410S), 69% (mutant enzyme E408P) Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
92
-
 ethanol mutant enzyme R91D/E408P/N410K, pH and temperature not specified in the publication Pseudomonas putida
93
-
 ethanol wild-type enzyme, pH and temperature not specified in the publication Pseudomonas putida