Cloned (Comment) | Organism |
---|---|
a plasmid construct is used to express PQQ-DH9. DH9. The expression host is a derivative Gluconobacter sp. strain of CHM43, which lacks the genes for PQQ-dependent glycerol dehydrogenase and the membrane-bound alcohol dehydrogenase and consequently has minimal ability to oxidize primary and secondary alcohols. The membranes of the transformant exhibited considerable d-arabitol dehydrogenase activity, whereas the reference strain did not, even if it had PQQ-DH9-encoding genes in the chromosome and harbored the empty vector. This suggests that PQQ-DH9 is not expressed in the genome | Gluconobacter sp. CHM43 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter sp. CHM43 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0031 | - |
substrate: threitol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.0052 | - |
substrate: D-arabinose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.01 | - |
substrate: trans-1,2-cyclohexanediol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.018 | - |
substrate: glycerol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.022 | - |
substrate: 1-cyclohexylethanol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.027 | - |
substrate: D-mannitol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.028 | - |
substrate: ribitol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.03 | - |
substrate: 2-hexanol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.035 | - |
substrate: isopropanol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.055 | - |
substrate: D-sorbitol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.065 | - |
substrate: 1,3-butanediol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.08 | - |
substrate: 1,2-butanediol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.084 | - |
substrate: cyclohexanol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.085 | - |
substrate: 2,4-pentanediol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.09 | - |
substrate: D-ribose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.1 | - |
substrate: D-lyxose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.12 | - |
substrate: D-xylose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.12 | - |
substrate: L-arabinose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.13 | - |
substrate: D-galactose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.13 | - |
substrate: D-gluconate, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.14 | - |
substrate: 2-butanol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.14 | - |
substrate: glyceraldehyde, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.17 | - |
substrate: D-arabitol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.19 | - |
substrate: meso-erythritol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.2 | - |
substrate: L-ribose, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.35 | - |
substrate: 1,2-cyclopentandiol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.4 | - |
substrate: 2,3-butanediol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
0.55 | - |
substrate: cis-1,2-cyclohexanediol, pH 5.0, 25°C | Gluconobacter sp. CHM43 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-butanediol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1,2-cyclopentandiol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1,3-butanediol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
1-cyclohexylethanol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
2,3-butanediol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
2,4-pentanediol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
2-butanol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
2-hexanol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
cis-1,2-cyclohexanediol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
cyclohexanol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
D-arabinose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
D-arabitol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
D-galactose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
D-gluconate + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
D-lyxose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
D-mannitol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
D-ribose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
D-sorbitol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
D-xylose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
glyceraldehyde + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
glycerol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
isopropanol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
L-arabinose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
L-ribose + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
meso-erythritol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
additional information | the enzyme oxidizes a wide variety of secondary alcohols but has higher KM-values than PQQ-dependent glycerol dehydrogenase with regard to linear substrates such as glycerol. Cyclic substrates such as cis-1,2-cyclohexanediol are readily oxidized | Gluconobacter sp. CHM43 | ? | - |
- |
|
ribitol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
threitol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? | |
trans-1,2-cyclohexanediol + phenazine methosulfate | - |
Gluconobacter sp. CHM43 | ? + reduced phenazine methosulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PQQ-DH9 | - |
Gluconobacter sp. CHM43 |
pyrroloquinoline quinone-dependent dehydrogenase | - |
Gluconobacter sp. CHM43 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | dependent on | Gluconobacter sp. CHM43 |