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Literature summary for 1.1.2.8 extracted from

  • Nguyen, T.; Naoki, K.; Kataoka, N.; Matsutani, M.; Ano, Y.; Adachi, O.; Matsushita, K.; Yakushi, T.
    Characterization of a cryptic, pyrroloquinoline quinone-dependent dehydrogenase of Gluconobacter sp. strain CHM43 (2021), Biosci. Biotechnol. Biochem., 85, 998-1004 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
a plasmid construct is used to express PQQ-DH9. DH9. The expression host is a derivative Gluconobacter sp. strain of CHM43, which lacks the genes for PQQ-dependent glycerol dehydrogenase and the membrane-bound alcohol dehydrogenase and consequently has minimal ability to oxidize primary and secondary alcohols. The membranes of the transformant exhibited considerable d-arabitol dehydrogenase activity, whereas the reference strain did not, even if it had PQQ-DH9-encoding genes in the chromosome and harbored the empty vector. This suggests that PQQ-DH9 is not expressed in the genome Gluconobacter sp. CHM43

Organism

Organism UniProt Comment Textmining
Gluconobacter sp. CHM43
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.0031
-
substrate: threitol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.0052
-
substrate: D-arabinose, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.01
-
substrate: trans-1,2-cyclohexanediol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.018
-
substrate: glycerol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.022
-
substrate: 1-cyclohexylethanol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.027
-
substrate: D-mannitol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.028
-
substrate: ribitol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.03
-
substrate: 2-hexanol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.035
-
substrate: isopropanol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.055
-
substrate: D-sorbitol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.065
-
substrate: 1,3-butanediol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.08
-
substrate: 1,2-butanediol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.084
-
substrate: cyclohexanol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.085
-
substrate: 2,4-pentanediol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.09
-
substrate: D-ribose, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.1
-
substrate: D-lyxose, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.12
-
substrate: D-xylose, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.12
-
substrate: L-arabinose, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.13
-
substrate: D-galactose, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.13
-
substrate: D-gluconate, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.14
-
substrate: 2-butanol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.14
-
substrate: glyceraldehyde, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.17
-
substrate: D-arabitol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.19
-
substrate: meso-erythritol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.2
-
substrate: L-ribose, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.35
-
substrate: 1,2-cyclopentandiol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.4
-
substrate: 2,3-butanediol, pH 5.0, 25┬░C Gluconobacter sp. CHM43
0.55
-
substrate: cis-1,2-cyclohexanediol, pH 5.0, 25┬░C Gluconobacter sp. CHM43

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-butanediol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
1,2-cyclopentandiol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
1,3-butanediol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
1-cyclohexylethanol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
2,3-butanediol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
2,4-pentanediol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
2-butanol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
2-hexanol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
cis-1,2-cyclohexanediol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
cyclohexanol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
D-arabinose + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
D-arabitol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
D-galactose + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
D-gluconate + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
D-lyxose + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
D-mannitol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
D-ribose + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
D-sorbitol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
D-xylose + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
glyceraldehyde + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
glycerol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
isopropanol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
L-arabinose + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
L-ribose + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
meso-erythritol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
additional information the enzyme oxidizes a wide variety of secondary alcohols but has higher KM-values than PQQ-dependent glycerol dehydrogenase with regard to linear substrates such as glycerol. Cyclic substrates such as cis-1,2-cyclohexanediol are readily oxidized Gluconobacter sp. CHM43 ?
-
-
ribitol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
threitol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?
trans-1,2-cyclohexanediol + phenazine methosulfate
-
Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate
-
?

Synonyms

Synonyms Comment Organism
PQQ-DH9
-
Gluconobacter sp. CHM43
pyrroloquinoline quinone-dependent dehydrogenase
-
Gluconobacter sp. CHM43

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone dependent on Gluconobacter sp. CHM43