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Literature summary for 1.1.2.8 extracted from

  • Takeda, K.; Ishida, T.; Igarashi, K.; Samejima, M.; Nakamura, N.; Ohno, H.
    Effect of amines as activators on the alcohol-oxidizing activity of pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase (2014), Biosci. Biotechnol. Biochem., 78, 1195-1198 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Butylamine
-
Pseudomonas putida
Dimethylamine
-
Pseudomonas putida
dipropylamine
-
Pseudomonas putida
ethylamine inhibitory at high concentrations Pseudomonas putida
heptylamine
-
Pseudomonas putida
Hexylamine
-
Pseudomonas putida
Isobutylamine slight activation Pseudomonas putida
methylamine
-
Pseudomonas putida
additional information the enzyme requires ammonia or primary amines as activators in in vitro assays with artificial electron acceptors, the enzyme is activated by various primary amines, dimethylamine, and trimethylamine. tert-Butylamine is a poor activator. The hydrophobic interaction contributes to the binding of amine activators to the binding site in the enzyme Pseudomonas putida
octylamine
-
Pseudomonas putida
pentylamine the alcohol oxidation activity of PpADH is strongly enhanced by pentylamine and the affinity for substrates is also improved by pentylamine as an activator, inhibitory at high concentrations Pseudomonas putida
Propylamine
-
Pseudomonas putida
triethylamine
-
Pseudomonas putida
trimethylamine
-
Pseudomonas putida
tripropylamine
-
Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
ethylamine activating at low concentrations, inhibitory at high concentrations Pseudomonas putida
pentylamine the alcohol oxidation activity of PpADH is strongly enhanced by pentylamine and the affinity for substrates is also improved by pentylamine as an activator, inhibitory at high concentrations Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
ethanol pH 9.0, temperature not specified in the publication, in presence of pentylamine Pseudomonas putida
0.21
-
ethanol pH 9.0, temperature not specified in the publication, in presence of ethylamine Pseudomonas putida
0.35
-
Propanol pH 9.0, temperature not specified in the publication, in presence of pentylamine Pseudomonas putida
0.43
-
Propanol pH 9.0, temperature not specified in the publication, in presence of ethylamine Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Pseudomonas putida
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida KT 2240
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + 2 cytochrome c
-
Pseudomonas putida ethanal + 2 reduced cytochrome c
-
?
ethanol + 2 cytochrome c
-
Pseudomonas putida KT 2240 ethanal + 2 reduced cytochrome c
-
?
additional information enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol Pseudomonas putida ?
-
?
additional information enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol Pseudomonas putida KT 2240 ?
-
?
propanol + 2 cytochrome c
-
Pseudomonas putida propanal + 2 reduced cytochrome c
-
?
propanol + 2 cytochrome c
-
Pseudomonas putida KT 2240 propanal + 2 reduced cytochrome c
-
?

Synonyms

Synonyms Comment Organism
PpADH
-
Pseudomonas putida
PQQ-ADH
-
Pseudomonas putida
pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24.8
-
ethanol pH 9.0, temperature not specified in the publication, in presence of ethylamine Pseudomonas putida
32.6
-
Propanol pH 9.0, temperature not specified in the publication, in presence of ethylamine Pseudomonas putida
56.6
-
ethanol pH 9.0, temperature not specified in the publication, in presence of pentylamine Pseudomonas putida
73.1
-
Propanol pH 9.0, temperature not specified in the publication, in presence of pentylamine Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
cytochrome c natural electron acceptor Pseudomonas putida
additional information enzyme assays are performed with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol Pseudomonas putida
pyrroloquinoline quinone prosthetic group, dependent on Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
37.6
-
pentylamine pH 9.0, temperature not specified in the publication Pseudomonas putida
112.5
-
ethylamine pH 9.0, temperature not specified in the publication Pseudomonas putida

General Information

General Information Comment Organism
evolution ADHs are categorized into three groups (type I, II, and III ADHs) according to their domain structure and localization. Type I ADHs have molecular and enzymatic properties that are very similar to those of methanol dehydrogenases, MDHs, but they have a low affinity for methanol. Type I ADHs, on the other hand, generally use ethylamine or methylamine as essential activators instead of ammonia Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
73
-
Propanol pH 9.0, temperature not specified in the publication, in presence of ethylamine Pseudomonas putida
115
-
ethanol pH 9.0, temperature not specified in the publication, in presence of ethylamine Pseudomonas putida
209
-
Propanol pH 9.0, temperature not specified in the publication, in presence of pentylamine Pseudomonas putida
395
-
ethanol pH 9.0, temperature not specified in the publication, in presence of pentylamine Pseudomonas putida