BRENDA - Enzyme Database show
show all sequences of 1.1.2.8

Isolation and characterization of Pseudomonas sp. strain capable of degrading diethylstilbestrol

Zhang, W.; Niu, Z.; Liao, C.; Chen, L.; Appl. Microbiol. Biotechnol. 97, 4095-4104 (2013)

Data extracted from this reference:

Application
Application
Commentary
Organism
environmental protection
potential application of Pseudomonas sp. strain J51 in the treatment of DES-contaminated freshwater and seawater environments
Pseudomonas sp.
Cloned(Commentary)
Commentary
Organism
sequence comparisons and phylogenetic tree, real-time reverse transcriptase PCR expression analysis
Pseudomonas mendocina
sequence comparisons and phylogenetic tree, real-time reverse transcriptase PCR expression analysis
Pseudomonas sp.
sequence comparisons and phylogenetic tree, real-time reverse transcriptase PCR expression analysis
Pseudomonas stutzeri
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
diethylstilbestrol + pyrroloquinoline quinone
Pseudomonas sp.
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
Pseudomonas stutzeri
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
Pseudomonas mendocina
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
Pseudomonas sp. J51
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
Pseudomonas stutzeri ATCC 14405
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
Pseudomonas mendocina YMP
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas mendocina
-
-
-
Pseudomonas mendocina YMP
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. J51
-
-
-
Pseudomonas stutzeri
-
-
-
Pseudomonas stutzeri ATCC 14405
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diethylstilbestrol + pyrroloquinoline quinone
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
741733
Pseudomonas sp.
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
741733
Pseudomonas stutzeri
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
741733
Pseudomonas mendocina
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
741733
Pseudomonas sp. J51
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
741733
Pseudomonas stutzeri ATCC 14405
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
741733
Pseudomonas mendocina YMP
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 68334, sequence calculation
Pseudomonas mendocina
?
x * 67951, sequence calculation
Pseudomonas stutzeri
Cofactor
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Pseudomonas mendocina
pyrroloquinoline quinone
-
Pseudomonas sp.
pyrroloquinoline quinone
-
Pseudomonas stutzeri
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas mendocina
sequence calculation
-
6.77
Pseudomonas stutzeri
sequence calculation
-
6.96
Application (protein specific)
Application
Commentary
Organism
environmental protection
potential application of Pseudomonas sp. strain J51 in the treatment of DES-contaminated freshwater and seawater environments
Pseudomonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
sequence comparisons and phylogenetic tree, real-time reverse transcriptase PCR expression analysis
Pseudomonas mendocina
sequence comparisons and phylogenetic tree, real-time reverse transcriptase PCR expression analysis
Pseudomonas sp.
sequence comparisons and phylogenetic tree, real-time reverse transcriptase PCR expression analysis
Pseudomonas stutzeri
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Pseudomonas mendocina
pyrroloquinoline quinone
-
Pseudomonas sp.
pyrroloquinoline quinone
-
Pseudomonas stutzeri
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
diethylstilbestrol + pyrroloquinoline quinone
Pseudomonas sp.
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
Pseudomonas stutzeri
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
Pseudomonas mendocina
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
Pseudomonas sp. J51
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
Pseudomonas stutzeri ATCC 14405
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
Pseudomonas mendocina YMP
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diethylstilbestrol + pyrroloquinoline quinone
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
741733
Pseudomonas sp.
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
741733
Pseudomonas stutzeri
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
741733
Pseudomonas mendocina
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
741733
Pseudomonas sp. J51
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
741733
Pseudomonas stutzeri ATCC 14405
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
741733
Pseudomonas mendocina YMP
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 68334, sequence calculation
Pseudomonas mendocina
?
x * 67951, sequence calculation
Pseudomonas stutzeri
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas mendocina
sequence calculation
-
6.77
Pseudomonas stutzeri
sequence calculation
-
6.96
Expression
Organism
Commentary
Expression
Pseudomonas sp.
the enzyme is induced by diethylstilbestrol, presence of diethylstilbestrol causes a 5.2fold increase in the mRNA level of the gene encoding quinoprotein alcohol dehydrogenase
up
General Information
General Information
Commentary
Organism
evolution
quinoprotein alcohol dehydrogenase usually occupies PQQ as a cofactor and belongs to the family of PQQ-dependent type I alcohol dehydrogenases, sequence comparisons and phylogenetic tree
Pseudomonas mendocina
evolution
quinoprotein alcohol dehydrogenase usually occupies PQQ as a cofactor and belongs to the family of PQQ-dependent type I alcohol dehydrogenases, sequence comparisons and phylogenetic tree
Pseudomonas sp.
evolution
quinoprotein alcohol dehydrogenase usually occupies PQQ as a cofactor and belongs to the family of PQQ-dependent type I alcohol dehydrogenases, sequence comparisons and phylogenetic tree
Pseudomonas stutzeri
physiological function
the enzyme is involved in diethylstilbestrol degradation
Pseudomonas mendocina
physiological function
the enzyme is involved in diethylstilbestrol degradation
Pseudomonas sp.
physiological function
the enzyme is involved in diethylstilbestrol degradation
Pseudomonas stutzeri
General Information (protein specific)
General Information
Commentary
Organism
evolution
quinoprotein alcohol dehydrogenase usually occupies PQQ as a cofactor and belongs to the family of PQQ-dependent type I alcohol dehydrogenases, sequence comparisons and phylogenetic tree
Pseudomonas mendocina
evolution
quinoprotein alcohol dehydrogenase usually occupies PQQ as a cofactor and belongs to the family of PQQ-dependent type I alcohol dehydrogenases, sequence comparisons and phylogenetic tree
Pseudomonas sp.
evolution
quinoprotein alcohol dehydrogenase usually occupies PQQ as a cofactor and belongs to the family of PQQ-dependent type I alcohol dehydrogenases, sequence comparisons and phylogenetic tree
Pseudomonas stutzeri
physiological function
the enzyme is involved in diethylstilbestrol degradation
Pseudomonas mendocina
physiological function
the enzyme is involved in diethylstilbestrol degradation
Pseudomonas sp.
physiological function
the enzyme is involved in diethylstilbestrol degradation
Pseudomonas stutzeri
Expression (protein specific)
Organism
Commentary
Expression
Pseudomonas sp.
the enzyme is induced by diethylstilbestrol, presence of diethylstilbestrol causes a 5.2fold increase in the mRNA level of the gene encoding quinoprotein alcohol dehydrogenase
up
Other publictions for EC 1.1.2.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742793
Good
Pyrroloquinoline quinone etha ...
Methylorubrum extorquens, Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
J. Bacteriol.
198
3109-3118
2016
2
-
1
-
1
-
-
-
-
3
1
6
-
16
-
-
1
-
-
1
-
-
8
1
1
-
-
-
1
-
-
3
-
-
-
2
-
1
3
-
1
-
-
-
-
-
-
3
1
6
-
-
-
1
-
1
-
-
8
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
743174
Simon
Analysis of the molecular res ...
Pseudomonas putida, Pseudomonas putida KT 2240
J. Proteomics
122
11-25
2015
-
-
-
-
1
-
-
-
1
-
-
8
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
2
-
-
-
-
-
2
-
-
8
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
2
4
-
-
-
742110
Takeda
Effect of amines as activator ...
Pseudomonas putida, Pseudomonas putida KT 2240
Biosci. Biotechnol. Biochem.
78
1195-1198
2014
15
-
-
-
-
-
2
4
1
1
-
-
-
44
-
-
-
-
-
-
-
-
6
-
1
-
-
4
1
-
-
3
2
-
-
15
-
-
3
-
-
-
-
2
2
4
1
1
-
-
-
-
-
-
-
-
-
-
6
-
1
-
-
4
1
-
-
-
-
1
1
-
4
4
741733
Zhang
Isolation and characterizatio ...
Pseudomonas mendocina, Pseudomonas mendocina YMP, Pseudomonas sp., Pseudomonas sp. J51, Pseudomonas stutzeri, Pseudomonas stutzeri ATCC 14405
Appl. Microbiol. Biotechnol.
97
4095-4104
2013
-
1
3
-
-
-
-
-
-
-
-
6
-
8
-
-
-
-
-
-
-
-
6
2
-
-
-
-
-
-
-
3
-
2
-
-
1
3
3
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
2
-
-
-
-
-
-
-
2
1
6
6
1
-
-
725041
Gomez-Manzo
The active (ADHa) and inactive ...
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus PAL5
FEMS Microbiol. Lett.
328
106-113
2012
-
-
-
-
-
-
-
-
1
1
6
-
-
3
-
-
1
-
-
1
-
-
-
2
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
1
6
-
-
-
-
1
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
724042
Kanchanarach
Characterization of thermotole ...
Acetobacter pasteurianus, Acetobacter pasteurianus IFO3191, Acetobacter pasteurianus MSU10, Acetobacter pasteurianus SKU1108
Appl. Microbiol. Biotechnol.
85
741-751
2010
-
-
1
-
-
-
-
-
-
-
-
16
1
16
-
-
1
-
-
1
-
-
16
-
2
-
-
-
1
-
-
1
-
-
-
-
-
5
8
-
-
-
-
-
-
-
-
-
-
16
8
-
-
8
-
8
-
-
16
-
8
-
-
-
8
-
-
-
-
1
8
-
-
-
725154
Masud
Cloning and functional analysi ...
Acetobacter pasteurianus, Acetobacter pasteurianus SKU1108
Int. J. Food Microbiol.
138
39-49
2010
1
-
-
-
9
-
-
-
1
1
-
2
-
6
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
9
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
674560
Kay
Structure of the pyrroloquinol ...
Pseudomonas aeruginosa
J. Biol. Chem.
281
1470-1476
2006
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676857
Kay
Substrate binding in quinoprot ...
Pseudomonas aeruginosa
Proc. Natl. Acad. Sci. USA
103
5267-5272
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389959
Keitel
X-ray structure of the quinopr ...
Pseudomonas aeruginosa
J. Mol. Biol.
297
961-974
2000
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389964
Diehl
Quinoprotein ethanol dehydroge ...
Pseudomonas aeruginosa
Eur. J. Biochem.
257
409-419
1998
-
-
1
1
1
-
-
-
-
-
2
-
-
1
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702114
Schrover
Quaternary structure of quinop ...
Pseudomonas aeruginosa
Biochem. J.
290 (Pt 1)
123-127
1993
-
-
-
-
-
-
-
-
-
1
3
-
-
1
-
-
1
-
-
-
1
-
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
3
-
-
-
-
1
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701600
Mutzel
-
Quinoprotein ethanol dehydroge ...
Pseudomonas aeruginosa
Agric. Biol. Chem.
55
1721-1726
1991
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705106
Stezowski
Preliminary X-ray crystallogra ...
Pseudomonas aeruginosa
J. Mol. Biol.
205
617-618
1989
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-